This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1hd8
From Proteopedia
(New page: 200px<br /> <applet load="1hd8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hd8, resolution 2.3Å" /> '''CRYSTAL STRUCTURE OF...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1HD8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.16.4 3.4.16.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HD8 OCA]]. | + | 1HD8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]]. Active as [[http://en.wikipedia.org/wiki/Serine-type_D-Ala-D-Ala_carboxypeptidase Serine-type D-Ala-D-Ala carboxypeptidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.16.4 3.4.16.4]]. Structure known Active Site: S44. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HD8 OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure of a deacylation-defective mutant of penicillin-binding protein 5 at 2.3-A resolution., Davies C, White SW, Nicholas RA, J Biol Chem. 2001 Jan 5;276(1):616-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10967102 10967102] | Crystal structure of a deacylation-defective mutant of penicillin-binding protein 5 at 2.3-A resolution., Davies C, White SW, Nicholas RA, J Biol Chem. 2001 Jan 5;276(1):616-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10967102 10967102] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| + | [[Category: Serine-type D-Ala-D-Ala carboxypeptidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Davies, C.]] | [[Category: Davies, C.]] | ||
| Line 22: | Line 23: | ||
[[Category: peptidoglycan synthesis]] | [[Category: peptidoglycan synthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:02:34 2007'' |
Revision as of 11:57, 30 October 2007
|
CRYSTAL STRUCTURE OF A DEACYLATION-DEFECTIVE MUTANT OF PENICILLIN-BINDING PROTEIN 5 AT 2.3 A RESOLUTION
Overview
Penicillin-binding protein 5 (PBP 5) of Escherichia coli functions as a, d-alanine carboxypeptidase, cleaving the C-terminal d-alanine residue from, cell wall peptides. Like all PBPs, PBP 5 forms a covalent acyl-enzyme, complex with beta-lactam antibiotics; however, PBP 5 is distinguished by, its high rate of deacylation of the acyl-enzyme complex (t(12), approximately 9 min). A Gly-105 --> Asp mutation in PBP 5 markedly impairs, this beta-lactamase activity (deacylation), with only minor effects on, acylation, and promotes accumulation of a covalent complex with peptide, substrates. To gain further insight into the catalytic mechanism of PBP 5, we determined the three-dimensional structure of the G105D mutant form of, soluble PBP 5 (termed sPBP 5') at 2.3 A resolution. The structure ... [(full description)]
About this Structure
1HD8 is a [Single protein] structure of sequence from [Escherichia coli]. Active as [Serine-type D-Ala-D-Ala carboxypeptidase], with EC number [3.4.16.4]. Structure known Active Site: S44. Full crystallographic information is available from [OCA].
Reference
Crystal structure of a deacylation-defective mutant of penicillin-binding protein 5 at 2.3-A resolution., Davies C, White SW, Nicholas RA, J Biol Chem. 2001 Jan 5;276(1):616-23. PMID:10967102
Page seeded by OCA on Tue Oct 30 14:02:34 2007
