1flm
From Proteopedia
(New page: 200px<br /><applet load="1flm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1flm, resolution 1.30Å" /> '''DIMER OF FMN-BINDING...) |
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| - | [[Image:1flm.gif|left|200px]]<br /><applet load="1flm" size=" | + | [[Image:1flm.gif|left|200px]]<br /><applet load="1flm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1flm, resolution 1.30Å" /> | caption="1flm, resolution 1.30Å" /> | ||
'''DIMER OF FMN-BINDING PROTEIN FROM DESULFOVIBRIO VULGARIS (MIYAZAKI F)'''<br /> | '''DIMER OF FMN-BINDING PROTEIN FROM DESULFOVIBRIO VULGARIS (MIYAZAKI F)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of FMN-binding protein (FMN-bp) from Desulfovibrio | + | The crystal structure of FMN-binding protein (FMN-bp) from Desulfovibrio vulgaris Miyazaki F was solved by the multiple isomorphous replacement method and refined to an R factor of 15.1% at 1.3 A resolution. FMN-bp exists in a dimeric form in the crystal, in contrast to the monomeric structure determined by NMR. R.m.s. deviations between the crystal structure and the solution structure are more than 2 A, which implies significant differences. There are some hydrophobic residues in the interface between the two monomers. In particular, Leu122 in the C-terminus has a close contact with the o-xylene moiety of FMN, while solvent molecules may cover the o-xylene moiety in the solution structure. |
==About this Structure== | ==About this Structure== | ||
| - | 1FLM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with FMN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1FLM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FLM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: fmn binding]] | [[Category: fmn binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:39:55 2008'' |
Revision as of 10:39, 21 February 2008
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DIMER OF FMN-BINDING PROTEIN FROM DESULFOVIBRIO VULGARIS (MIYAZAKI F)
Overview
The crystal structure of FMN-binding protein (FMN-bp) from Desulfovibrio vulgaris Miyazaki F was solved by the multiple isomorphous replacement method and refined to an R factor of 15.1% at 1.3 A resolution. FMN-bp exists in a dimeric form in the crystal, in contrast to the monomeric structure determined by NMR. R.m.s. deviations between the crystal structure and the solution structure are more than 2 A, which implies significant differences. There are some hydrophobic residues in the interface between the two monomers. In particular, Leu122 in the C-terminus has a close contact with the o-xylene moiety of FMN, while solvent molecules may cover the o-xylene moiety in the solution structure.
About this Structure
1FLM is a Single protein structure of sequence from Desulfovibrio vulgaris with as ligand. Full crystallographic information is available from OCA.
Reference
How do the x-ray structure and the NMR structure of FMN-binding protein differ?, Suto K, Kawagoe K, Shibata N, Morimoto Y, Higuchi Y, Kitamura M, Nakaya T, Yasuoka N, Acta Crystallogr D Biol Crystallogr. 2000 Mar;56(Pt 3):368-71. PMID:10713530
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