1flh
From Proteopedia
(New page: 200px<br /> <applet load="1flh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1flh, resolution 2.45Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1flh.gif|left|200px]]<br /> | + | [[Image:1flh.gif|left|200px]]<br /><applet load="1flh" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1flh" size=" | + | |
caption="1flh, resolution 2.45Å" /> | caption="1flh, resolution 2.45Å" /> | ||
'''CRYSTAL STRUCTURE OF HUMAN UROPEPSIN AT 2.45 A RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF HUMAN UROPEPSIN AT 2.45 A RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The molecular structure of human uropepsin, an aspartic proteinase from | + | The molecular structure of human uropepsin, an aspartic proteinase from the urine produced in the form of pepsinogen A in the gastric mucosa, has been determined by molecular replacement using human pepsin as the search model. Crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.99, b = 75.56, c = 89.90 A. Crystallographic refinement led to an R factor of 0.161 at 2.45 A resolution. The positions of 2437 non-H protein atoms in 326 residues have been determined and the model contains 143 water molecules. The structure is bilobal, consisting of two predominantly beta-sheet lobes which, as observed in other aspartic proteinases, are related by a pseudo-twofold axis. A model of the uropepsin-pepstatin complex has been constructed based on the high-resolution crystal structure of pepsin complexed with pepstatin. |
==About this Structure== | ==About this Structure== | ||
| - | 1FLH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Pepsin_A Pepsin A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.1 3.4.23.1] Full crystallographic information is available from [http:// | + | 1FLH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Pepsin_A Pepsin A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.1 3.4.23.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FLH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Canduri, F.]] | [[Category: Canduri, F.]] | ||
[[Category: Fadel, V.]] | [[Category: Fadel, V.]] | ||
| - | [[Category: Gomes, R | + | [[Category: Gomes, R A.S.]] |
[[Category: Hial, V.]] | [[Category: Hial, V.]] | ||
| - | [[Category: Jr., W | + | [[Category: Jr., W F.De Azevedo.]] |
| - | [[Category: Lorenzi, C | + | [[Category: Lorenzi, C C.B.]] |
| - | [[Category: Neto, J | + | [[Category: Neto, J R.]] |
| - | [[Category: Teodoro, L | + | [[Category: Teodoro, L G.V L.]] |
[[Category: acid proteinase]] | [[Category: acid proteinase]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:39:56 2008'' |
Revision as of 10:40, 21 February 2008
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CRYSTAL STRUCTURE OF HUMAN UROPEPSIN AT 2.45 A RESOLUTION
Overview
The molecular structure of human uropepsin, an aspartic proteinase from the urine produced in the form of pepsinogen A in the gastric mucosa, has been determined by molecular replacement using human pepsin as the search model. Crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.99, b = 75.56, c = 89.90 A. Crystallographic refinement led to an R factor of 0.161 at 2.45 A resolution. The positions of 2437 non-H protein atoms in 326 residues have been determined and the model contains 143 water molecules. The structure is bilobal, consisting of two predominantly beta-sheet lobes which, as observed in other aspartic proteinases, are related by a pseudo-twofold axis. A model of the uropepsin-pepstatin complex has been constructed based on the high-resolution crystal structure of pepsin complexed with pepstatin.
About this Structure
1FLH is a Single protein structure of sequence from Homo sapiens. Active as Pepsin A, with EC number 3.4.23.1 Full crystallographic information is available from OCA.
Reference
Structure of human uropepsin at 2.45 A resolution., Canduri F, Teodoro LG, Fadel V, Lorenzi CC, Hial V, Gomes RA, Neto JR, de Azevedo WF Jr, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1560-70. Epub 2001, Oct 25. PMID:11679720
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