1fnw
From Proteopedia
(New page: 200px<br /><applet load="1fnw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fnw, resolution 3.9Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1fnw.gif|left|200px]]<br /><applet load="1fnw" size=" | + | [[Image:1fnw.gif|left|200px]]<br /><applet load="1fnw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fnw, resolution 3.9Å" /> | caption="1fnw, resolution 3.9Å" /> | ||
'''CRYSTAL STRUCTURE OF STREPTOCOCCAL PYROGENIC EXOTOXIN A'''<br /> | '''CRYSTAL STRUCTURE OF STREPTOCOCCAL PYROGENIC EXOTOXIN A'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The streptococcal pyrogenic toxins A, B, and C (SPEA, SPEB, and SPEC) are | + | The streptococcal pyrogenic toxins A, B, and C (SPEA, SPEB, and SPEC) are responsible for the fever, rash, and other toxicities associated with scarlet fever and streptococcal toxic shock syndrome. This role, together with the ubiquity of diseases caused by Streptococcus pyogenes, have prompted structural analyses of SPEA by several groups. Papageorgiou et al. (1999) have recently reported the structure of SPEA crystallized in the absence of zinc. Zinc has been shown to be important in the ability of some staphylococcal and streptococcal toxins to stimulate proliferation of CD4+ T-cells. Since cadmium is more electron dense than zinc and typically binds interchangeably, we grew crystals in the presence of 10 mM CdCl2. Crystals have been obtained in three space groups, and the structure in the P2(1)2(1)2(1) crystal form has been refined to 1.9 A resolution. The structural analysis revealed an identical tetramer as well as a novel tetrahedral cluster of cadmium in all three crystal forms on a disulfide loop encompassing residues 87-98. No cadmium was bound at the site homologous to the zinc site in staphylococcal enterotoxins C (SECs) despite the high structural homology between SPEA and SECs. Subsequent soaking of crystals grown in the presence of cadmium in 10 mM ZnCl2 showed that zinc binds in this site (indicating it can discriminate between zinc and cadmium ions) using the three ligands (Asp77, His106, and His110) homologous to the SECs plus a fourth ligand (Glu33). |
==About this Structure== | ==About this Structure== | ||
| - | 1FNW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1FNW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FNW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptococcus pyogenes]] | [[Category: Streptococcus pyogenes]] | ||
| - | [[Category: Earhart, C | + | [[Category: Earhart, C A.]] |
| - | [[Category: Ohlendorf, D | + | [[Category: Ohlendorf, D H.]] |
[[Category: Roggiani, M.]] | [[Category: Roggiani, M.]] | ||
| - | [[Category: Schlivert, P | + | [[Category: Schlivert, P M.]] |
| - | [[Category: Vath, G | + | [[Category: Vath, G M.]] |
[[Category: CD]] | [[Category: CD]] | ||
[[Category: exotoxin a]] | [[Category: exotoxin a]] | ||
[[Category: superantigen]] | [[Category: superantigen]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:40:42 2008'' |
Revision as of 10:40, 21 February 2008
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CRYSTAL STRUCTURE OF STREPTOCOCCAL PYROGENIC EXOTOXIN A
Overview
The streptococcal pyrogenic toxins A, B, and C (SPEA, SPEB, and SPEC) are responsible for the fever, rash, and other toxicities associated with scarlet fever and streptococcal toxic shock syndrome. This role, together with the ubiquity of diseases caused by Streptococcus pyogenes, have prompted structural analyses of SPEA by several groups. Papageorgiou et al. (1999) have recently reported the structure of SPEA crystallized in the absence of zinc. Zinc has been shown to be important in the ability of some staphylococcal and streptococcal toxins to stimulate proliferation of CD4+ T-cells. Since cadmium is more electron dense than zinc and typically binds interchangeably, we grew crystals in the presence of 10 mM CdCl2. Crystals have been obtained in three space groups, and the structure in the P2(1)2(1)2(1) crystal form has been refined to 1.9 A resolution. The structural analysis revealed an identical tetramer as well as a novel tetrahedral cluster of cadmium in all three crystal forms on a disulfide loop encompassing residues 87-98. No cadmium was bound at the site homologous to the zinc site in staphylococcal enterotoxins C (SECs) despite the high structural homology between SPEA and SECs. Subsequent soaking of crystals grown in the presence of cadmium in 10 mM ZnCl2 showed that zinc binds in this site (indicating it can discriminate between zinc and cadmium ions) using the three ligands (Asp77, His106, and His110) homologous to the SECs plus a fourth ligand (Glu33).
About this Structure
1FNW is a Single protein structure of sequence from Streptococcus pyogenes with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of streptococcal pyrogenic exotoxin A reveals a novel metal cluster., Earhart CA, Vath GM, Roggiani M, Schlievert PM, Ohlendorf DH, Protein Sci. 2000 Sep;9(9):1847-51. PMID:11045630
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