1juy
From Proteopedia
(New page: 200px<br /> <applet load="1juy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1juy, resolution 2.5Å" /> '''REFINED CRYSTAL STRU...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1JUY is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG, PI, GDP, H5P and HDA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JUY OCA]]. | + | 1JUY is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG, PI, GDP, H5P and HDA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4]]. Structure known Active Sites: ASP, GNS and IMP. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JUY OCA]]. |
==Reference== | ==Reference== | ||
Refined crystal structure of adenylosuccinate synthetase from Escherichia coli complexed with hydantocidin 5'-phosphate, GDP, HPO4(2-), Mg2+, and hadacidin., Poland BW, Lee SF, Subramanian MV, Siehl DL, Anderson RJ, Fromm HJ, Honzatko RB, Biochemistry. 1996 Dec 10;35(49):15753-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8961938 8961938] | Refined crystal structure of adenylosuccinate synthetase from Escherichia coli complexed with hydantocidin 5'-phosphate, GDP, HPO4(2-), Mg2+, and hadacidin., Poland BW, Lee SF, Subramanian MV, Siehl DL, Anderson RJ, Fromm HJ, Honzatko RB, Biochemistry. 1996 Dec 10;35(49):15753-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8961938 8961938] | ||
| + | [[Category: Adenylosuccinate synthase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: purine biosynthesis]] | [[Category: purine biosynthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:03:55 2007'' |
Revision as of 11:59, 30 October 2007
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REFINED CRYSTAL STRUCTURE OF ADENYLOSUCCINATE SYNTHETASE FROM ESCHERICHIA COLI COMPLEXED WITH HYDANTOCIDIN 5'-PHOSPHATE GDP, HPO4(2-), MG2+, AND HADACIDIN
Overview
A crystal structure of adenylosuccinate synthetase from Escherichia coli, complexed with 5'-phosphate, GDP, HPO4(2-), Mg2+, and hadacidin at 100 K, has been refined to an Rfactor of 0.195 against data to 2.6 A resolution., Bond lengths and angles deviate from expected values by 0.012 A and 1.86, degrees, respectively. Lys 16 and backbone amides 15-17 and 42 interact, with the phosphates of GDP, while Ser 414, Asp 333, and backbone amides, 331 and 416 interact with the base. Mg2+ is octahedrally coordinated., Oxygen atoms from GDP, phosphate, and hadacidin define the equatorial, plane of coordination of the Mg2+, while backbone carbonyl 40 and the side, chain of Asp 13 are the apical ligands. HPO4(2-) hydrogen bonds with Lys, 16, His 41, backbone amides 13, 40, and 224, and the base moiety ... [(full description)]
About this Structure
1JUY is a [Single protein] structure of sequence from [Escherichia coli] with MG, PI, GDP, H5P and HDA as [ligands]. Active as [Adenylosuccinate synthase], with EC number [6.3.4.4]. Structure known Active Sites: ASP, GNS and IMP. Full crystallographic information is available from [OCA].
Reference
Refined crystal structure of adenylosuccinate synthetase from Escherichia coli complexed with hydantocidin 5'-phosphate, GDP, HPO4(2-), Mg2+, and hadacidin., Poland BW, Lee SF, Subramanian MV, Siehl DL, Anderson RJ, Fromm HJ, Honzatko RB, Biochemistry. 1996 Dec 10;35(49):15753-9. PMID:8961938
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