1o7d
From Proteopedia
(New page: 200px<br /> <applet load="1o7d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1o7d, resolution 2.70Å" /> '''THE STRUCTURE OF TH...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1O7D is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with NAG, ZN, SO4 and TRS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O7D OCA]]. | + | 1O7D is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with NAG, ZN, SO4 and TRS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Alpha-mannosidase Alpha-mannosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.24 3.2.1.24]]. Structure known Active Site: ACT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O7D OCA]]. |
==Reference== | ==Reference== | ||
The structure of bovine lysosomal alpha-mannosidase suggests a novel mechanism for low-pH activation., Heikinheimo P, Helland R, Leiros HK, Leiros I, Karlsen S, Evjen G, Ravelli R, Schoehn G, Ruigrok R, Tollersrud OK, McSweeney S, Hough E, J Mol Biol. 2003 Mar 28;327(3):631-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12634058 12634058] | The structure of bovine lysosomal alpha-mannosidase suggests a novel mechanism for low-pH activation., Heikinheimo P, Helland R, Leiros HK, Leiros I, Karlsen S, Evjen G, Ravelli R, Schoehn G, Ruigrok R, Tollersrud OK, McSweeney S, Hough E, J Mol Biol. 2003 Mar 28;327(3):631-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12634058 12634058] | ||
| + | [[Category: Alpha-mannosidase]] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: lysosomal]] | [[Category: lysosomal]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:04:25 2007'' |
Revision as of 11:59, 30 October 2007
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THE STRUCTURE OF THE BOVINE LYSOSOMAL A-MANNOSIDASE SUGGESTS A NOVEL MECHANISM FOR LOW PH ACTIVATION
Overview
Lysosomal alpha-mannosidase (LAM: EC 3.2.1.24) belongs to the, sequence-based glycoside hydrolase family 38 (GH38). Two other mammalian, GH38 members, Golgi alpha-mannosidase II (GIIAM) and cytosolic, alpha-mannosidase, are expressed in all tissues. In humans, cattle, cat, and guinea pig, lack of lysosomal alpha-mannosidase activity causes the, autosomal recessive disease alpha-mannosidosis. Here, we describe the, three-dimensional structure of bovine lysosomal alpha-mannosidase (bLAM), at 2.7A resolution and confirm the solution state dimer by electron, microscopy. We present the first structure of a mammalian GH38 enzyme that, offers indications for the signal areas for mannose phosphorylation, suggests a previously undetected mechanism of low-pH activation and, provides a template for ... [(full description)]
About this Structure
1O7D is a [Single protein] structure of sequence from [Bos taurus] with NAG, ZN, SO4 and TRS as [ligands]. Active as [Alpha-mannosidase], with EC number [3.2.1.24]. Structure known Active Site: ACT. Full crystallographic information is available from [OCA].
Reference
The structure of bovine lysosomal alpha-mannosidase suggests a novel mechanism for low-pH activation., Heikinheimo P, Helland R, Leiros HK, Leiros I, Karlsen S, Evjen G, Ravelli R, Schoehn G, Ruigrok R, Tollersrud OK, McSweeney S, Hough E, J Mol Biol. 2003 Mar 28;327(3):631-44. PMID:12634058
Page seeded by OCA on Tue Oct 30 14:04:25 2007
Categories: Alpha-mannosidase | Bos taurus | Single protein | Evjen, G. | Heikinheimo, P. | Helland, R. | Hough, E. | Karlsen, S. | Leiros, H.S. | Leiros, I. | Mcsweeney, S. | Ravelli, R. | Ruigrok, R. | Schoehn, G. | Tollersrud, O.K. | NAG | SO4 | TRS | ZN | A-mannosidase | Glycosyl hydrolase family 38 | Lysosomal
