1fts
From Proteopedia
(New page: 200px<br /><applet load="1fts" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fts, resolution 2.2Å" /> '''SIGNAL RECOGNITION PA...) |
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| - | [[Image:1fts.jpg|left|200px]]<br /><applet load="1fts" size=" | + | [[Image:1fts.jpg|left|200px]]<br /><applet load="1fts" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fts, resolution 2.2Å" /> | caption="1fts, resolution 2.2Å" /> | ||
'''SIGNAL RECOGNITION PARTICLE RECEPTOR FROM E. COLI'''<br /> | '''SIGNAL RECOGNITION PARTICLE RECEPTOR FROM E. COLI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | FtsY is the docking protein or SR alpha homologue in E. coli. It is | + | FtsY is the docking protein or SR alpha homologue in E. coli. It is involved in targeting secretory proteins to the cytoplasmic membrane by interacting with the signal recognition particle, controlled by guanosine 5'-triphosphate. Two different constructs have been used in crystallization studies: the full-length protein and a truncated fragment with a his-tag at the C terminus. Only the second construct resulted in crystals suitable for x-ray diffraction. The crystals belong to the monoclinic space group P2(1) with cell dimensions a = 32.20 A, b = 79.57 A, c = 59.21 A, and beta = 94.45, and contain one molecule per asymmetric unit. At cryogenic temperatures the crystals diffract to a resolution limit of 2.5 A by using a rotating anode, and beyond 1.8 A by using synchrotron radiation. |
==About this Structure== | ==About this Structure== | ||
| - | 1FTS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1FTS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FTS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: signal recognition particle receptor]] | [[Category: signal recognition particle receptor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:42:40 2008'' |
Revision as of 10:42, 21 February 2008
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SIGNAL RECOGNITION PARTICLE RECEPTOR FROM E. COLI
Overview
FtsY is the docking protein or SR alpha homologue in E. coli. It is involved in targeting secretory proteins to the cytoplasmic membrane by interacting with the signal recognition particle, controlled by guanosine 5'-triphosphate. Two different constructs have been used in crystallization studies: the full-length protein and a truncated fragment with a his-tag at the C terminus. Only the second construct resulted in crystals suitable for x-ray diffraction. The crystals belong to the monoclinic space group P2(1) with cell dimensions a = 32.20 A, b = 79.57 A, c = 59.21 A, and beta = 94.45, and contain one molecule per asymmetric unit. At cryogenic temperatures the crystals diffract to a resolution limit of 2.5 A by using a rotating anode, and beyond 1.8 A by using synchrotron radiation.
About this Structure
1FTS is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli., Montoya G, Svensson C, Luirink J, Sinning I, Proteins. 1997 Jun;28(2):285-8. PMID:9188744
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