1fvi
From Proteopedia
(New page: 200px<br /> <applet load="1fvi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fvi, resolution 2.0Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1fvi.gif|left|200px]]<br /> | + | [[Image:1fvi.gif|left|200px]]<br /><applet load="1fvi" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1fvi" size=" | + | |
caption="1fvi, resolution 2.0Å" /> | caption="1fvi, resolution 2.0Å" /> | ||
'''CRYSTAL STRUCTURE OF CHLORELLA VIRUS DNA LIGASE-ADENYLATE'''<br /> | '''CRYSTAL STRUCTURE OF CHLORELLA VIRUS DNA LIGASE-ADENYLATE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Chlorella virus DNA ligase is the smallest eukaryotic ATP-dependent ligase | + | Chlorella virus DNA ligase is the smallest eukaryotic ATP-dependent ligase known; it has an intrinsic nick-sensing function and suffices for yeast cell growth. Here, we report the 2.0 A crystal structure of the covalent ligase-AMP reaction intermediate. The conformation of the adenosine nucleoside and contacts between the enzyme and the ribose sugar have undergone a significant change compared to complexes of T7 ligase with ATP or mRNA capping enzyme with GTP. The conformational switch allows the 3' OH of AMP to coordinate directly the 5' PO(4) of the nick. The structure explains why nick sensing is restricted to adenylated ligase and why the 5' phosphate is required for DNA binding. We identify a metal binding site on ligase-adenylate and propose a mechanism of nick recognition and catalysis supported by mutational data. |
==About this Structure== | ==About this Structure== | ||
| - | 1FVI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlorella_virus Chlorella virus] with SO4 and AMP as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1FVI with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb55_1.html DNA Ligase]]. Full crystallographic information is available from [http:// | + | 1FVI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlorella_virus Chlorella virus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=AMP:'>AMP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1FVI with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb55_1.html DNA Ligase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FVI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: DNA Ligase]] | [[Category: DNA Ligase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Nikolov, D | + | [[Category: Nikolov, D B.]] |
[[Category: Odell, M.]] | [[Category: Odell, M.]] | ||
[[Category: Shuman, S.]] | [[Category: Shuman, S.]] | ||
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[[Category: adenylated dna ligase]] | [[Category: adenylated dna ligase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:43:02 2008'' |
Revision as of 10:43, 21 February 2008
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CRYSTAL STRUCTURE OF CHLORELLA VIRUS DNA LIGASE-ADENYLATE
Overview
Chlorella virus DNA ligase is the smallest eukaryotic ATP-dependent ligase known; it has an intrinsic nick-sensing function and suffices for yeast cell growth. Here, we report the 2.0 A crystal structure of the covalent ligase-AMP reaction intermediate. The conformation of the adenosine nucleoside and contacts between the enzyme and the ribose sugar have undergone a significant change compared to complexes of T7 ligase with ATP or mRNA capping enzyme with GTP. The conformational switch allows the 3' OH of AMP to coordinate directly the 5' PO(4) of the nick. The structure explains why nick sensing is restricted to adenylated ligase and why the 5' phosphate is required for DNA binding. We identify a metal binding site on ligase-adenylate and propose a mechanism of nick recognition and catalysis supported by mutational data.
About this Structure
1FVI is a Single protein structure of sequence from Chlorella virus with and as ligands. The following page contains interesting information on the relation of 1FVI with [DNA Ligase]. Full crystallographic information is available from OCA.
Reference
Crystal structure of eukaryotic DNA ligase-adenylate illuminates the mechanism of nick sensing and strand joining., Odell M, Sriskanda V, Shuman S, Nikolov DB, Mol Cell. 2000 Nov;6(5):1183-93. PMID:11106756
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