1fyo
From Proteopedia
(New page: 200px<br /><applet load="1fyo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fyo" /> '''EUKARYOTIC DECODING REGION A-SITE RNA'''<br ...) |
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'''EUKARYOTIC DECODING REGION A-SITE RNA'''<br /> | '''EUKARYOTIC DECODING REGION A-SITE RNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The aminoglycoside antibiotics target a region of highly conserved | + | The aminoglycoside antibiotics target a region of highly conserved nucleotides in the aminoacyl-tRNA site (A site) of 16 S RNA on the 30 S subunit. The structures of a prokaryotic decoding region A-site oligonucleotide free in solution and bound to the aminoglycosides paromomycin and gentamicin C1A have been determined. Here, the structure of a eukaryotic decoding region A-site oligonucleotide has been determined using homonuclear and heteronuclear NMR spectroscopy, and compared to the unbound prokaryotic rRNA structure. The two structures are similar, with a U1406-U1495 base-pair, a C1407-G1494 Watson-Crick base-pair, and a G1408-A1493 base-pair instead of the A1408-A1493 base-pair of the prokaryotic structure. The two structures differ in the orientation of the 1408 position with respect to A1493; G1408 is rotated toward the major groove, which is the binding pocket for aminoglycosides. The structures also differ in the stacking geometry of G1494 on A1493, which could have slight long-range conformational effects. |
==About this Structure== | ==About this Structure== | ||
| - | 1FYO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1FYO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FYO OCA]. |
==Reference== | ==Reference== | ||
Structure of a eukaryotic decoding region A-site RNA., Lynch SR, Puglisi JD, J Mol Biol. 2001 Mar 9;306(5):1023-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11237616 11237616] | Structure of a eukaryotic decoding region A-site RNA., Lynch SR, Puglisi JD, J Mol Biol. 2001 Mar 9;306(5):1023-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11237616 11237616] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Lynch, S | + | [[Category: Lynch, S R.]] |
| - | [[Category: Puglisi, J | + | [[Category: Puglisi, J D.]] |
[[Category: bulged a]] | [[Category: bulged a]] | ||
[[Category: g-a base pair]] | [[Category: g-a base pair]] | ||
[[Category: stem-internal loop-stem-tetraloop rna]] | [[Category: stem-internal loop-stem-tetraloop rna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:44:02 2008'' |
Revision as of 10:44, 21 February 2008
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EUKARYOTIC DECODING REGION A-SITE RNA
Overview
The aminoglycoside antibiotics target a region of highly conserved nucleotides in the aminoacyl-tRNA site (A site) of 16 S RNA on the 30 S subunit. The structures of a prokaryotic decoding region A-site oligonucleotide free in solution and bound to the aminoglycosides paromomycin and gentamicin C1A have been determined. Here, the structure of a eukaryotic decoding region A-site oligonucleotide has been determined using homonuclear and heteronuclear NMR spectroscopy, and compared to the unbound prokaryotic rRNA structure. The two structures are similar, with a U1406-U1495 base-pair, a C1407-G1494 Watson-Crick base-pair, and a G1408-A1493 base-pair instead of the A1408-A1493 base-pair of the prokaryotic structure. The two structures differ in the orientation of the 1408 position with respect to A1493; G1408 is rotated toward the major groove, which is the binding pocket for aminoglycosides. The structures also differ in the stacking geometry of G1494 on A1493, which could have slight long-range conformational effects.
About this Structure
1FYO is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of a eukaryotic decoding region A-site RNA., Lynch SR, Puglisi JD, J Mol Biol. 2001 Mar 9;306(5):1023-35. PMID:11237616
Page seeded by OCA on Thu Feb 21 12:44:02 2008
