1ovw
From Proteopedia
(New page: 200px<br /> <applet load="1ovw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ovw, resolution 2.7Å" /> '''ENDOGLUCANASE I COMP...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1OVW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]] with NAG and DP5 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OVW OCA]]. | + | 1OVW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]] with NAG and DP5 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Cellulase Cellulase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Structure known Active Sites: CTA, CTB, CTC and CTD. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OVW OCA]]. |
==Reference== | ==Reference== | ||
Structure of the Fusarium oxysporum endoglucanase I with a nonhydrolyzable substrate analogue: substrate distortion gives rise to the preferred axial orientation for the leaving group., Sulzenbacher G, Driguez H, Henrissat B, Schulein M, Davies GJ, Biochemistry. 1996 Dec 3;35(48):15280-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8952478 8952478] | Structure of the Fusarium oxysporum endoglucanase I with a nonhydrolyzable substrate analogue: substrate distortion gives rise to the preferred axial orientation for the leaving group., Sulzenbacher G, Driguez H, Henrissat B, Schulein M, Davies GJ, Biochemistry. 1996 Dec 3;35(48):15280-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8952478 8952478] | ||
| + | [[Category: Cellulase]] | ||
[[Category: Fusarium oxysporum]] | [[Category: Fusarium oxysporum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:06:00 2007'' |
Revision as of 12:01, 30 October 2007
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ENDOGLUCANASE I COMPLEXED WITH NON-HYDROLYSABLE SUBSTRATE ANALOGUE
Overview
Endoglucanase I (EG I) is a cellulase, from glycosyl hydrolase family 7, which cleaves the beta-1,4 linkages of cellulose with overall retention of, configuration. The structure of the EG I from Fusarium oxysproum, complexed to a nonhydrolyzable thiooligosaccharide substrate analogue, has, been determined by X-ray crystallography at a resolution of 2.7 A, utilizing the 4-fold noncrystallographic symmetry present in the, asymmetric unit. The electron density map clearly reveals the presence of, three glucosyl units of the inhibitor, consistent with the known number of, sugar-binding subsites, located at the active site of the enzyme in the, -2, -1, and +1 subsites, i.e., actually spanning the point of enzymatic, cleavage. The pyranose ring at the point of potential enzymatic cleavage, is ... [(full description)]
About this Structure
1OVW is a [Single protein] structure of sequence from [Fusarium oxysporum] with NAG and DP5 as [ligands]. Active as [Cellulase], with EC number [3.2.1.4]. Structure known Active Sites: CTA, CTB, CTC and CTD. Full crystallographic information is available from [OCA].
Reference
Structure of the Fusarium oxysporum endoglucanase I with a nonhydrolyzable substrate analogue: substrate distortion gives rise to the preferred axial orientation for the leaving group., Sulzenbacher G, Driguez H, Henrissat B, Schulein M, Davies GJ, Biochemistry. 1996 Dec 3;35(48):15280-7. PMID:8952478
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