1g12
From Proteopedia
(New page: 200px<br /><applet load="1g12" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g12, resolution 1.6Å" /> '''ZINC PEPTIDASE FROM G...) |
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| - | [[Image:1g12.gif|left|200px]]<br /><applet load="1g12" size=" | + | [[Image:1g12.gif|left|200px]]<br /><applet load="1g12" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1g12, resolution 1.6Å" /> | caption="1g12, resolution 1.6Å" /> | ||
'''ZINC PEPTIDASE FROM GRIFOLA FRONDOSA'''<br /> | '''ZINC PEPTIDASE FROM GRIFOLA FRONDOSA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Crystal structures of a peptidyl-Lys metalloendopeptidase (MEP) from the | + | Crystal structures of a peptidyl-Lys metalloendopeptidase (MEP) from the edible mushroom Grifola frondosa (GfMEP) were solved in four crystal forms. This represents the first structure of the new family 'aspzincins' with a novel active-site architecture. The active site is composed of two helices and a loop region and includes the HExxH and GTxDxxYG motifs conserved among aspzincins. His117, His121 and Asp130 coordinate to the catalytic zinc ligands. An electrostatically negative region composed of Asp154 and Glu157 attracts a positively charged Lys side chain of a substrate in a specific manner. A Tyr133 side chain located on the S1' pocket had different configurations in two crystal forms and was not observed in the other crystal forms. The flexible Tyr133 plays two roles in the enzymatic function of GfMEP. The first is to provide a hydrophobic environment with Phe83 in order to accommodate the alkyl part of the Lys side chain of a substrate and the second is as a 'proton donor' to the oxyanion of the tetrahedral transition state to stabilize the reaction transition state. |
==About this Structure== | ==About this Structure== | ||
| - | 1G12 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Grifola_frondosa Grifola frondosa] with MAN and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peptidyl-Lys_metalloendopeptidase Peptidyl-Lys metalloendopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.20 3.4.24.20] Full crystallographic information is available from [http:// | + | 1G12 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Grifola_frondosa Grifola frondosa] with <scene name='pdbligand=MAN:'>MAN</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peptidyl-Lys_metalloendopeptidase Peptidyl-Lys metalloendopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.20 3.4.24.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G12 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc cordinate]] | [[Category: zinc cordinate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:44:50 2008'' |
Revision as of 10:44, 21 February 2008
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ZINC PEPTIDASE FROM GRIFOLA FRONDOSA
Overview
Crystal structures of a peptidyl-Lys metalloendopeptidase (MEP) from the edible mushroom Grifola frondosa (GfMEP) were solved in four crystal forms. This represents the first structure of the new family 'aspzincins' with a novel active-site architecture. The active site is composed of two helices and a loop region and includes the HExxH and GTxDxxYG motifs conserved among aspzincins. His117, His121 and Asp130 coordinate to the catalytic zinc ligands. An electrostatically negative region composed of Asp154 and Glu157 attracts a positively charged Lys side chain of a substrate in a specific manner. A Tyr133 side chain located on the S1' pocket had different configurations in two crystal forms and was not observed in the other crystal forms. The flexible Tyr133 plays two roles in the enzymatic function of GfMEP. The first is to provide a hydrophobic environment with Phe83 in order to accommodate the alkyl part of the Lys side chain of a substrate and the second is as a 'proton donor' to the oxyanion of the tetrahedral transition state to stabilize the reaction transition state.
About this Structure
1G12 is a Single protein structure of sequence from Grifola frondosa with and as ligands. Active as Peptidyl-Lys metalloendopeptidase, with EC number 3.4.24.20 Full crystallographic information is available from OCA.
Reference
Structure of a new 'aspzincin' metalloendopeptidase from Grifola frondosa: implications for the catalytic mechanism and substrate specificity based on several different crystal forms., Hori T, Kumasaka T, Yamamoto M, Nonaka N, Tanaka N, Hashimoto Y, Ueki U, Takio K, Acta Crystallogr D Biol Crystallogr. 2001 Mar;57(Pt 3):361-8. PMID:11223512
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