1g19
From Proteopedia
(New page: 200px<br /><applet load="1g19" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g19, resolution 3.00Å" /> '''STRUCTURE OF RECA PR...) |
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| - | [[Image:1g19.gif|left|200px]]<br /><applet load="1g19" size=" | + | [[Image:1g19.gif|left|200px]]<br /><applet load="1g19" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1g19, resolution 3.00Å" /> | caption="1g19, resolution 3.00Å" /> | ||
'''STRUCTURE OF RECA PROTEIN'''<br /> | '''STRUCTURE OF RECA PROTEIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Sequencing of the complete genome of Mycobacterium tuberculosis, combined | + | Sequencing of the complete genome of Mycobacterium tuberculosis, combined with the rapidly increasing need to improve tuberculosis management through better drugs and vaccines, has initiated extensive research on several key proteins from the pathogen. RecA, a ubiquitous multifunctional protein, is a key component of the processes of homologous genetic recombination and DNA repair. Structural knowledge of MtRecA is imperative for a full understanding of both these activities and any ensuing application. The crystal structure of MtRecA, presented here, has six molecules in the unit cell forming a 6(1) helical filament with a deep groove capable of binding DNA. The observed weakening in the higher order aggregation of filaments into bundles may have implications for recombination in mycobacteria. The structure of the complex reveals the atomic interactions of ADP-AlF(4), an ATP analogue, with the P-loop-containing binding pocket. The structures explain reduced levels of interactions of MtRecA with ATP, despite sharing the same fold, topology and high sequence similarity with EcRecA. The formation of a helical filament with a deep groove appears to be an inherent property of MtRecA. The histidine in loop L1 appears to be positioned appropriately for DNA interaction. |
==About this Structure== | ==About this Structure== | ||
| - | 1G19 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] Full crystallographic information is available from [http:// | + | 1G19 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G19 OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chandra, N | + | [[Category: Chandra, N R.]] |
[[Category: Datta, S.]] | [[Category: Datta, S.]] | ||
[[Category: Ganesh, N.]] | [[Category: Ganesh, N.]] | ||
[[Category: Muniyappa, K.]] | [[Category: Muniyappa, K.]] | ||
| - | [[Category: Prabu, M | + | [[Category: Prabu, M M.]] |
| - | [[Category: TBSGC, TB | + | [[Category: TBSGC, TB Structural Genomics Consortium.]] |
| - | [[Category: Vaze, M | + | [[Category: Vaze, M B.]] |
[[Category: Vijayan, M.]] | [[Category: Vijayan, M.]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
| Line 31: | Line 31: | ||
[[Category: tbsgc]] | [[Category: tbsgc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:44:56 2008'' |
Revision as of 10:44, 21 February 2008
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STRUCTURE OF RECA PROTEIN
Overview
Sequencing of the complete genome of Mycobacterium tuberculosis, combined with the rapidly increasing need to improve tuberculosis management through better drugs and vaccines, has initiated extensive research on several key proteins from the pathogen. RecA, a ubiquitous multifunctional protein, is a key component of the processes of homologous genetic recombination and DNA repair. Structural knowledge of MtRecA is imperative for a full understanding of both these activities and any ensuing application. The crystal structure of MtRecA, presented here, has six molecules in the unit cell forming a 6(1) helical filament with a deep groove capable of binding DNA. The observed weakening in the higher order aggregation of filaments into bundles may have implications for recombination in mycobacteria. The structure of the complex reveals the atomic interactions of ADP-AlF(4), an ATP analogue, with the P-loop-containing binding pocket. The structures explain reduced levels of interactions of MtRecA with ATP, despite sharing the same fold, topology and high sequence similarity with EcRecA. The formation of a helical filament with a deep groove appears to be an inherent property of MtRecA. The histidine in loop L1 appears to be positioned appropriately for DNA interaction.
About this Structure
1G19 is a Single protein structure of sequence from Mycobacterium tuberculosis with as ligand. Active as Deleted entry, with EC number 3.4.99.37 Full crystallographic information is available from OCA.
Reference
Crystal structures of Mycobacterium tuberculosis RecA and its complex with ADP-AlF(4): implications for decreased ATPase activity and molecular aggregation., Datta S, Prabu MM, Vaze MB, Ganesh N, Chandra NR, Muniyappa K, Vijayan M, Nucleic Acids Res. 2000 Dec 15;28(24):4964-73. PMID:11121488
Page seeded by OCA on Thu Feb 21 12:44:56 2008
Categories: Deleted entry | Mycobacterium tuberculosis | Single protein | Chandra, N R. | Datta, S. | Ganesh, N. | Muniyappa, K. | Prabu, M M. | TBSGC, TB Structural Genomics Consortium. | Vaze, M B. | Vijayan, M. | PO4 | Dna-repair | Protein structure initiative | Psi | Recombination | Structural genomics | Tb structural genomics consortium | Tbsgc
