1bkj
From Proteopedia
(New page: 200px<br /> <applet load="1bkj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bkj, resolution 1.8Å" /> '''NADPH:FMN OXIDOREDUC...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1BKJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]] with PO4 and FMN as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entry 1CUM. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.8.1 1.6.8.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BKJ OCA]]. | + | 1BKJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]] with PO4 and FMN as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entry 1CUM. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_1.5.1.29 Transferred entry: 1.5.1.29]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.8.1 1.6.8.1]]. Structure known Active Sites: ASA and ASB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BKJ OCA]]. |
==Reference== | ==Reference== | ||
Flavin reductase P: structure of a dimeric enzyme that reduces flavin., Tanner JJ, Lei B, Tu SC, Krause KL, Biochemistry. 1996 Oct 22;35(42):13531-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8885832 8885832] | Flavin reductase P: structure of a dimeric enzyme that reduces flavin., Tanner JJ, Lei B, Tu SC, Krause KL, Biochemistry. 1996 Oct 22;35(42):13531-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8885832 8885832] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Transferred entry: 1.5.1.29]] | ||
[[Category: Vibrio harveyi]] | [[Category: Vibrio harveyi]] | ||
[[Category: Krause, K.L.]] | [[Category: Krause, K.L.]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:06:13 2007'' |
Revision as of 12:01, 30 October 2007
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NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI
Overview
We report the structure of an NADPH:FMN oxidoreductase (flavin reductase, P) that is involved in bioluminescence by providing reduced FMN to, luciferase. The 1.8 A crystal structure of flavin reductase P from Vibrio, harveyi was solved by multiple isomorphous replacement and reveals that, the enzyme is a unique dimer of interlocking subunits, with 9352 A2 of, surface area buried in the dimer interface. Each subunit comprises two, domains. The first domain consists of a four-stranded antiparallel, beta-sheet flanked by helices on either side. The second domain reaches, out from one subunit and embraces the other subunit and is responsible for, interlocking the two subunits. Our structure explains why flavin reductase, P is specific for FMN as cofactor. FMN is recognized and tightly bound ... [(full description)]
About this Structure
1BKJ is a [Single protein] structure of sequence from [Vibrio harveyi] with PO4 and FMN as [ligands]. This structure superseeds the now removed PDB entry 1CUM. Active as [Transferred entry: 1.5.1.29], with EC number [1.6.8.1]. Structure known Active Sites: ASA and ASB. Full crystallographic information is available from [OCA].
Reference
Flavin reductase P: structure of a dimeric enzyme that reduces flavin., Tanner JJ, Lei B, Tu SC, Krause KL, Biochemistry. 1996 Oct 22;35(42):13531-9. PMID:8885832
Page seeded by OCA on Tue Oct 30 14:06:13 2007
Categories: Single protein | Transferred entry: 1.5.1.29 | Vibrio harveyi | Krause, K.L. | Lei, B. | TU, S.C. | Tanner, J.J. | FMN | PO4 | Flavoprotein | Luminescence | Nadp | Oxidoreductase
