1g2c
From Proteopedia
(New page: 200px<br /><applet load="1g2c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g2c, resolution 2.30Å" /> '''HUMAN RESPIRATORY SY...) |
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| - | [[Image:1g2c.gif|left|200px]]<br /><applet load="1g2c" size=" | + | [[Image:1g2c.gif|left|200px]]<br /><applet load="1g2c" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1g2c, resolution 2.30Å" /> | caption="1g2c, resolution 2.30Å" /> | ||
'''HUMAN RESPIRATORY SYNCYTIAL VIRUS FUSION PROTEIN CORE'''<br /> | '''HUMAN RESPIRATORY SYNCYTIAL VIRUS FUSION PROTEIN CORE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Human respiratory syncytial virus (HRSV) is a major cause of a number of | + | Human respiratory syncytial virus (HRSV) is a major cause of a number of severe respiratory diseases, including bronchiolitis and pneumonia, in infants and young children. The HRSV F protein, a glycoprotein essential for viral entry, is a primary target for vaccine and drug development. Two heptad-repeat regions within the HRSV F sequence were predicted by the computer program learncoil-vmf. These regions are thought to form trimer-of-hairpins-like structures, similar to those found in the fusion proteins of several enveloped viruses. The hairpin structure likely brings the viral and cellular membranes into close apposition, thereby facilitating membrane fusion and subsequent viral entry. Here, we show that peptides, denoted HR-N and HR-C, corresponding to the heptad-repeat regions from the N-terminal and C-terminal segments of the HRSV F protein, respectively, form a stable alpha-helical trimer of heterodimers. The HRSV N/C complex was crystallized and its x-ray structure was determined at 2.3-A resolution. As anticipated, the complex is a six-helix bundle in which the HR-N peptides form a three-stranded, central coiled coil, and the HR-C peptides pack in an antiparallel manner into hydrophobic grooves on the coiled-coil surface. There is remarkable structural similarity between the HRSV N/C complex and the fusion protein core of other viruses, including HIV-1 gp41. In addition, earlier work has shown that HRSV HR-C peptides, like the HIV-1 gp41 C peptides, inhibit viral infection. Thus, drug discovery and vaccine development strategies aimed at inhibiting viral entry by blocking hairpin formation may be applied to the inhibition of HRSV. |
==About this Structure== | ==About this Structure== | ||
| - | 1G2C is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus_a Human respiratory syncytial virus a]. Full crystallographic information is available from [http:// | + | 1G2C is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus_a Human respiratory syncytial virus a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G2C OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Human respiratory syncytial virus a]] | [[Category: Human respiratory syncytial virus a]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Kim, P | + | [[Category: Kim, P S.]] |
| - | [[Category: Malashkevich, V | + | [[Category: Malashkevich, V N.]] |
[[Category: Singh, M.]] | [[Category: Singh, M.]] | ||
[[Category: Zhao, X.]] | [[Category: Zhao, X.]] | ||
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[[Category: pneumovirus]] | [[Category: pneumovirus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:45:15 2008'' |
Revision as of 10:45, 21 February 2008
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HUMAN RESPIRATORY SYNCYTIAL VIRUS FUSION PROTEIN CORE
Overview
Human respiratory syncytial virus (HRSV) is a major cause of a number of severe respiratory diseases, including bronchiolitis and pneumonia, in infants and young children. The HRSV F protein, a glycoprotein essential for viral entry, is a primary target for vaccine and drug development. Two heptad-repeat regions within the HRSV F sequence were predicted by the computer program learncoil-vmf. These regions are thought to form trimer-of-hairpins-like structures, similar to those found in the fusion proteins of several enveloped viruses. The hairpin structure likely brings the viral and cellular membranes into close apposition, thereby facilitating membrane fusion and subsequent viral entry. Here, we show that peptides, denoted HR-N and HR-C, corresponding to the heptad-repeat regions from the N-terminal and C-terminal segments of the HRSV F protein, respectively, form a stable alpha-helical trimer of heterodimers. The HRSV N/C complex was crystallized and its x-ray structure was determined at 2.3-A resolution. As anticipated, the complex is a six-helix bundle in which the HR-N peptides form a three-stranded, central coiled coil, and the HR-C peptides pack in an antiparallel manner into hydrophobic grooves on the coiled-coil surface. There is remarkable structural similarity between the HRSV N/C complex and the fusion protein core of other viruses, including HIV-1 gp41. In addition, earlier work has shown that HRSV HR-C peptides, like the HIV-1 gp41 C peptides, inhibit viral infection. Thus, drug discovery and vaccine development strategies aimed at inhibiting viral entry by blocking hairpin formation may be applied to the inhibition of HRSV.
About this Structure
1G2C is a Protein complex structure of sequences from Human respiratory syncytial virus a. Full crystallographic information is available from OCA.
Reference
Structural characterization of the human respiratory syncytial virus fusion protein core., Zhao X, Singh M, Malashkevich VN, Kim PS, Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14172-7. PMID:11106388
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