1g2r
From Proteopedia
(New page: 200px<br /><applet load="1g2r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g2r, resolution 1.35Å" /> '''Structure of Cytosol...) |
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| - | [[Image:1g2r.gif|left|200px]]<br /><applet load="1g2r" size=" | + | [[Image:1g2r.gif|left|200px]]<br /><applet load="1g2r" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1g2r, resolution 1.35Å" /> | caption="1g2r, resolution 1.35Å" /> | ||
'''Structure of Cytosolic Protein of Unknown Function Coded by Gene from NUSA/INFB Region, a YlxR Homologue'''<br /> | '''Structure of Cytosolic Protein of Unknown Function Coded by Gene from NUSA/INFB Region, a YlxR Homologue'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the YlxR protein of unknown function from Streptococcus | + | The structure of the YlxR protein of unknown function from Streptococcus pneumonia was determined to 1.35 A. YlxR is expressed from the nusA/infB operon in bacteria and belongs to a small protein family (COG2740) that shares a conserved sequence motif GRGA(Y/W). The family shows no significant amino-acid sequence similarity with other proteins. Three-wavelength diffraction MAD data were collected to 1.7 A from orthorhombic crystals using synchrotron radiation and the structure was determined using a semi-automated approach. The YlxR structure resembles a two-layer alpha/beta sandwich with the overall shape of a cylinder and shows no structural homology to proteins of known structure. Structural analysis revealed that the YlxR structure represents a new protein fold that belongs to the alpha-beta plait superfamily. The distribution of the electrostatic surface potential shows a large positively charged patch on one side of the protein, a feature often found in nucleic acid-binding proteins. Three sulfate ions bind to this positively charged surface. Analysis of potential binding sites uncovered several substantial clefts, with the largest spanning 3/4 of the protein. A similar distribution of binding sites and a large sharply bent cleft are observed in RNA-binding proteins that are unrelated in sequence and structure. It is proposed that YlxR is an RNA-binding protein. |
==About this Structure== | ==About this Structure== | ||
| - | 1G2R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1G2R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G2R OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Gornicki, P.]] | [[Category: Gornicki, P.]] | ||
[[Category: Joachimiak, A.]] | [[Category: Joachimiak, A.]] | ||
| - | [[Category: MCSG, Midwest | + | [[Category: MCSG, Midwest Center for Structural Genomics.]] |
[[Category: Maj, L.]] | [[Category: Maj, L.]] | ||
[[Category: Osipiuk, J.]] | [[Category: Osipiuk, J.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:45:24 2008'' |
Revision as of 10:45, 21 February 2008
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Structure of Cytosolic Protein of Unknown Function Coded by Gene from NUSA/INFB Region, a YlxR Homologue
Overview
The structure of the YlxR protein of unknown function from Streptococcus pneumonia was determined to 1.35 A. YlxR is expressed from the nusA/infB operon in bacteria and belongs to a small protein family (COG2740) that shares a conserved sequence motif GRGA(Y/W). The family shows no significant amino-acid sequence similarity with other proteins. Three-wavelength diffraction MAD data were collected to 1.7 A from orthorhombic crystals using synchrotron radiation and the structure was determined using a semi-automated approach. The YlxR structure resembles a two-layer alpha/beta sandwich with the overall shape of a cylinder and shows no structural homology to proteins of known structure. Structural analysis revealed that the YlxR structure represents a new protein fold that belongs to the alpha-beta plait superfamily. The distribution of the electrostatic surface potential shows a large positively charged patch on one side of the protein, a feature often found in nucleic acid-binding proteins. Three sulfate ions bind to this positively charged surface. Analysis of potential binding sites uncovered several substantial clefts, with the largest spanning 3/4 of the protein. A similar distribution of binding sites and a large sharply bent cleft are observed in RNA-binding proteins that are unrelated in sequence and structure. It is proposed that YlxR is an RNA-binding protein.
About this Structure
1G2R is a Single protein structure of sequence from Streptococcus pneumoniae with as ligand. Full crystallographic information is available from OCA.
Reference
Streptococcus pneumonia YlxR at 1.35 A shows a putative new fold., Osipiuk J, Gornicki P, Maj L, Dementieva I, Laskowski R, Joachimiak A, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1747-51. Epub 2001, Oct 25. PMID:11679764
Page seeded by OCA on Thu Feb 21 12:45:24 2008
Categories: Single protein | Streptococcus pneumoniae | Gornicki, P. | Joachimiak, A. | MCSG, Midwest Center for Structural Genomics. | Maj, L. | Osipiuk, J. | SO4 | Hypothetical | Mcsg | Midwest center for structural genomics | Nusa-infb operon | Protein structure initiative | Psi | Structural genomics
