1g3x
From Proteopedia
(New page: 200px<br /><applet load="1g3x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g3x, resolution 2.700Å" /> '''INTERCALATION OF AN...) |
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| - | [[Image:1g3x.gif|left|200px]]<br /><applet load="1g3x" size=" | + | [[Image:1g3x.gif|left|200px]]<br /><applet load="1g3x" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1g3x, resolution 2.700Å" /> | caption="1g3x, resolution 2.700Å" /> | ||
'''INTERCALATION OF AN 9ACRIDINE-PEPTIDE DRUG IN A DNA DODECAMER'''<br /> | '''INTERCALATION OF AN 9ACRIDINE-PEPTIDE DRUG IN A DNA DODECAMER'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We present the crystal structure of an acridine drug derivatized at carbon | + | We present the crystal structure of an acridine drug derivatized at carbon 9, [N(alpha)-(9-acridinoyl)-tetraarginine], intercalated within the dodecamer [d(CGCGAATTCGCG)](2). The presence of a lateral chain at the central carbon 9 atom differentiates this compound from most acridine drugs hitherto studied, which are usually derivatized at carbon 4. The DNA:drug interaction we observe differs from that observed in previous studies, which primarily involves shorter, mainly hexameric sequences, in two important regards: the acridine intercalates within an AA/TT base step, rather than within a CG/CG base step; and the binding site is located at the center of the sequence, rather than at one end of the duplex. In addition, we observe a novel crystal packing arrangement, with six dodecamer duplexes and seven hydrated magnesium ions in the asymmetric unit of a large (66.5 x 68.4 x 77.4 A(3)) unit cell in space group P2(1)2(1)2(1). The duplexes are organized in layers parallel to the ab plane, with consecutive layers crossing each other at right angles. |
==About this Structure== | ==About this Structure== | ||
| - | 1G3X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1G3X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G3X OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Malinina, L.]] | [[Category: Malinina, L.]] | ||
[[Category: Soler-Lopez, M.]] | [[Category: Soler-Lopez, M.]] | ||
| - | [[Category: Subirana, J | + | [[Category: Subirana, J A.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: dna]] | [[Category: dna]] | ||
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[[Category: x-ray structure]] | [[Category: x-ray structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:45:47 2008'' |
Revision as of 10:45, 21 February 2008
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INTERCALATION OF AN 9ACRIDINE-PEPTIDE DRUG IN A DNA DODECAMER
Overview
We present the crystal structure of an acridine drug derivatized at carbon 9, [N(alpha)-(9-acridinoyl)-tetraarginine], intercalated within the dodecamer [d(CGCGAATTCGCG)](2). The presence of a lateral chain at the central carbon 9 atom differentiates this compound from most acridine drugs hitherto studied, which are usually derivatized at carbon 4. The DNA:drug interaction we observe differs from that observed in previous studies, which primarily involves shorter, mainly hexameric sequences, in two important regards: the acridine intercalates within an AA/TT base step, rather than within a CG/CG base step; and the binding site is located at the center of the sequence, rather than at one end of the duplex. In addition, we observe a novel crystal packing arrangement, with six dodecamer duplexes and seven hydrated magnesium ions in the asymmetric unit of a large (66.5 x 68.4 x 77.4 A(3)) unit cell in space group P2(1)2(1)2(1). The duplexes are organized in layers parallel to the ab plane, with consecutive layers crossing each other at right angles.
About this Structure
1G3X is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Intercalation of an acridine-peptide drug in an AA/TT base step in the crystal structure of [d(CGCGAATTCGCG)](2) with six duplexes and seven Mg(2+) ions in the asymmetric unit., Malinina L, Soler-Lopez M, Aymami J, Subirana JA, Biochemistry. 2002 Jul 30;41(30):9341-8. PMID:12135355
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