1g43
From Proteopedia
(New page: 200px<br /><applet load="1g43" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g43, resolution 2.20Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1g43.jpg|left|200px]]<br /><applet load="1g43" size=" | + | [[Image:1g43.jpg|left|200px]]<br /><applet load="1g43" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1g43, resolution 2.20Å" /> | caption="1g43, resolution 2.20Å" /> | ||
'''CRYSTAL STRUCTURE OF A FAMILY IIIA CBD FROM CLOSTRIDIUM CELLULOLYTICUM'''<br /> | '''CRYSTAL STRUCTURE OF A FAMILY IIIA CBD FROM CLOSTRIDIUM CELLULOLYTICUM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the family IIIa cellulose-binding domain (CBD) | + | The crystal structure of the family IIIa cellulose-binding domain (CBD) from the cellulosomal scaffoldin subunit (CipC) of Clostridium cellulolyticum has been determined. The structure reveals a nine-stranded jelly-roll topology which exhibits distinctive structural elements consistent with family III CBDs that bind crystalline cellulose. These include a well conserved calcium-binding site, a putative cellulose-binding surface and a conserved shallow groove of unknown function. The CipC CBD structure is very similar to the previously elucidated family IIIa CBD from the CipA scaffoldin of C. thermocellum, with some minor differences. The CipC CBD structure was also compared with other previously described CBD structures from families IIIc and IV derived from the endoglucanases of Thermomonospora fusca and Cellulomonas fimi, respectively. The possible functional consequences of structural similarities and differences in the shallow groove and cellulose-binding faces among various CBD families and subfamilies are discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1G43 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_cellulolyticum Clostridium cellulolyticum] with CA and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1G43 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_cellulolyticum Clostridium cellulolyticum] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G43 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Clostridium cellulolyticum]] | [[Category: Clostridium cellulolyticum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bayer, E | + | [[Category: Bayer, E A.]] |
[[Category: Belaich, A.]] | [[Category: Belaich, A.]] | ||
| - | [[Category: Belaich, J | + | [[Category: Belaich, J P.]] |
[[Category: Frolow, F.]] | [[Category: Frolow, F.]] | ||
[[Category: Lamed, R.]] | [[Category: Lamed, R.]] | ||
[[Category: Pages, S.]] | [[Category: Pages, S.]] | ||
| - | [[Category: Shimon, L | + | [[Category: Shimon, L J.W.]] |
[[Category: Shoham, Y.]] | [[Category: Shoham, Y.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: beta-sandwich]] | [[Category: beta-sandwich]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:45:52 2008'' |
Revision as of 10:45, 21 February 2008
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CRYSTAL STRUCTURE OF A FAMILY IIIA CBD FROM CLOSTRIDIUM CELLULOLYTICUM
Overview
The crystal structure of the family IIIa cellulose-binding domain (CBD) from the cellulosomal scaffoldin subunit (CipC) of Clostridium cellulolyticum has been determined. The structure reveals a nine-stranded jelly-roll topology which exhibits distinctive structural elements consistent with family III CBDs that bind crystalline cellulose. These include a well conserved calcium-binding site, a putative cellulose-binding surface and a conserved shallow groove of unknown function. The CipC CBD structure is very similar to the previously elucidated family IIIa CBD from the CipA scaffoldin of C. thermocellum, with some minor differences. The CipC CBD structure was also compared with other previously described CBD structures from families IIIc and IV derived from the endoglucanases of Thermomonospora fusca and Cellulomonas fimi, respectively. The possible functional consequences of structural similarities and differences in the shallow groove and cellulose-binding faces among various CBD families and subfamilies are discussed.
About this Structure
1G43 is a Single protein structure of sequence from Clostridium cellulolyticum with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of a family IIIa scaffoldin CBD from the cellulosome of Clostridium cellulolyticum at 2.2 A resolution., Shimon LJ, Pages S, Belaich A, Belaich JP, Bayer EA, Lamed R, Shoham Y, Frolow F, Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1560-8. PMID:11092922
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