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1oh4

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(New page: 200px<br /> <applet load="1oh4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oh4, resolution 1.35&Aring;" /> '''STRUCTURAL AND THER...)
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==About this Structure==
==About this Structure==
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1OH4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]] with GLA, CA, SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OH4 OCA]].
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1OH4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]] with GLA, CA, SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OH4 OCA]].
==Reference==
==Reference==
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[[Category: mannan]]
[[Category: mannan]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 21:35:01 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:07:49 2007''

Revision as of 12:03, 30 October 2007

Image:1oh4.gif

1oh4, resolution 1.35Å

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STRUCTURAL AND THERMODYNAMIC DISSECTION OF SPECIFIC MANNAN RECOGNITION BY A CARBOHYDRATE-BINDING MODULE

Overview

The C-terminal 176 amino acids of a Thermotoga maritima mannanase (Man5), constitute a carbohydrate binding module (CBM) that has been classified, into CBM family 27. The isolated CBM27 domain, named TmCBM27, binds, tightly (K(a)s 10(5)-10(6) M(-1)) to beta-1, 4-mannooligosaccharides, carob galactomannan, and konjac glucomannan, but not to cellulose, (insoluble and soluble) or soluble birchwood xylan. The X-ray crystal, structures of native TmCBM27, a TmCBM27-mannohexaose complex, and a, TmCBM27-6(3),6(4)-alpha-D-galactosyl-mannopentaose complex at 2.0 A, 1.6, A, and 1.35 A, respectively, reveal the basis of TmCBM27's specificity for, mannans. In particular, the latter complex, which is the first structure, of a CBM in complex with a branched plant cell wall polysaccharide, illustrates ... [(full description)]

About this Structure

1OH4 is a [Single protein] structure of sequence from [Thermotoga maritima] with GLA, CA, SO4 and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate binding module, TmCBM27., Boraston AB, Revett TJ, Boraston CM, Nurizzo D, Davies GJ, Structure. 2003 Jun;11(6):665-75. PMID:12791255

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