1gco
From Proteopedia
(New page: 200px<br /> <applet load="1gco" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gco, resolution 1.70Å" /> '''CRYSTAL STRUCTURE O...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1gco.gif|left|200px]]<br /> | + | [[Image:1gco.gif|left|200px]]<br /><applet load="1gco" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1gco" size=" | + | |
caption="1gco, resolution 1.70Å" /> | caption="1gco, resolution 1.70Å" /> | ||
'''CRYSTAL STRUCTURE OF GLUCOSE DEHYDROGENASE COMPLEXED WITH NAD+'''<br /> | '''CRYSTAL STRUCTURE OF GLUCOSE DEHYDROGENASE COMPLEXED WITH NAD+'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of glucose dehydrogenase (GlcDH) from Bacillus | + | The crystal structure of glucose dehydrogenase (GlcDH) from Bacillus megaterium IWG3 has been determined to an R-factor of 17.9% at 1.7 A resolution. The enzyme consists of four identical subunits, which are similar to those of other short-chain reductases/dehydrogenases (SDRs) in their overall folding and subunit architecture, although cofactor binding sites and subunit interactions differ. Whereas a pair of basic residues is well conserved among NADP(+)-preferring SDRs, only Arg39 was found around the adenine ribose moiety of GlcDH. This suggests that one basic amino acid is enough to determine the coenzyme specificity. The four subunits are interrelated by three mutually perpendicular diad axes (P, Q, and R). While subunit interactions through the P-axis for GlcDH are not so different from those of the other SDRs, those through the Q-axis differ significantly. GlcDH was found to have weaker hydrophobic interactions in the Q-interface. Moreover, GlcDH lacks the salt bridge that stabilizes the subunit interaction in the Q-interface in the other SDRs. Hydrogen bonds between Q-axis related subunits are also less common than in the other SDRs. The GlcDH tetramer dissociates into inactive monomers at pH 9.0, which can be attributed mainly to the weakness of the Q-axis interface. |
==About this Structure== | ==About this Structure== | ||
| - | 1GCO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1GCO with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb77_1.html Glucose Oxidase]]. Active as [http://en.wikipedia.org/wiki/Glucose_1-dehydrogenase Glucose 1-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.47 1.1.1.47] Full crystallographic information is available from [http:// | + | 1GCO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium] with <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1GCO with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb77_1.html Glucose Oxidase]]. Active as [http://en.wikipedia.org/wiki/Glucose_1-dehydrogenase Glucose 1-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.47 1.1.1.47] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GCO OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of glucose dehydrogenase from Bacillus megaterium IWG3 at 1.7 A resolution., Yamamoto K, Kurisu G, Kusunoki M, Tabata S, Urabe I, Osaki S, J Biochem | + | Crystal structure of glucose dehydrogenase from Bacillus megaterium IWG3 at 1.7 A resolution., Yamamoto K, Kurisu G, Kusunoki M, Tabata S, Urabe I, Osaki S, J Biochem. 2001 Feb;129(2):303-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11173533 11173533] |
[[Category: Bacillus megaterium]] | [[Category: Bacillus megaterium]] | ||
[[Category: Glucose 1-dehydrogenase]] | [[Category: Glucose 1-dehydrogenase]] | ||
| Line 26: | Line 25: | ||
[[Category: short-chain dehydrogenase/reductase]] | [[Category: short-chain dehydrogenase/reductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:48:40 2008'' |
Revision as of 10:48, 21 February 2008
|
CRYSTAL STRUCTURE OF GLUCOSE DEHYDROGENASE COMPLEXED WITH NAD+
Overview
The crystal structure of glucose dehydrogenase (GlcDH) from Bacillus megaterium IWG3 has been determined to an R-factor of 17.9% at 1.7 A resolution. The enzyme consists of four identical subunits, which are similar to those of other short-chain reductases/dehydrogenases (SDRs) in their overall folding and subunit architecture, although cofactor binding sites and subunit interactions differ. Whereas a pair of basic residues is well conserved among NADP(+)-preferring SDRs, only Arg39 was found around the adenine ribose moiety of GlcDH. This suggests that one basic amino acid is enough to determine the coenzyme specificity. The four subunits are interrelated by three mutually perpendicular diad axes (P, Q, and R). While subunit interactions through the P-axis for GlcDH are not so different from those of the other SDRs, those through the Q-axis differ significantly. GlcDH was found to have weaker hydrophobic interactions in the Q-interface. Moreover, GlcDH lacks the salt bridge that stabilizes the subunit interaction in the Q-interface in the other SDRs. Hydrogen bonds between Q-axis related subunits are also less common than in the other SDRs. The GlcDH tetramer dissociates into inactive monomers at pH 9.0, which can be attributed mainly to the weakness of the Q-axis interface.
About this Structure
1GCO is a Single protein structure of sequence from Bacillus megaterium with as ligand. The following page contains interesting information on the relation of 1GCO with [Glucose Oxidase]. Active as Glucose 1-dehydrogenase, with EC number 1.1.1.47 Full crystallographic information is available from OCA.
Reference
Crystal structure of glucose dehydrogenase from Bacillus megaterium IWG3 at 1.7 A resolution., Yamamoto K, Kurisu G, Kusunoki M, Tabata S, Urabe I, Osaki S, J Biochem. 2001 Feb;129(2):303-12. PMID:11173533
Page seeded by OCA on Thu Feb 21 12:48:40 2008
