1ggi
From Proteopedia
(New page: 200px<br /> <applet load="1ggi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ggi, resolution 2.8Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1ggi.gif|left|200px]]<br /> | + | [[Image:1ggi.gif|left|200px]]<br /><applet load="1ggi" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ggi" size=" | + | |
caption="1ggi, resolution 2.8Å" /> | caption="1ggi, resolution 2.8Å" /> | ||
'''CRYSTAL STRUCTURE OF AN HIV-1 NEUTRALIZING ANTIBODY 50.1 IN COMPLEX WITH ITS V3 LOOP PEPTIDE ANTIGEN'''<br /> | '''CRYSTAL STRUCTURE OF AN HIV-1 NEUTRALIZING ANTIBODY 50.1 IN COMPLEX WITH ITS V3 LOOP PEPTIDE ANTIGEN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the Fab fragment of a human immunodeficiency | + | The crystal structure of the Fab fragment of a human immunodeficiency virus type 1 (HIV-1) neutralizing monoclonal antibody Fab has been determined at 2.8 A resolution in complex with a linear 16-residue peptide from the third hypervariable region (V3) of gp120. The first 9 residues of the peptide are ordered in the electron density maps, and their conformation is in partial agreement with the beta-strand-type II beta-turn structure predicted for this portion of the V3 loop. Notably, several of the peptide residues that are well conserved among different HIV-1 isolates contact a nonpolar 25-A-long groove in the antibody-combining site. The largely extended structure of the peptide differs from the beta-turns seen as the primary determinants in other published anti-peptide Fab structures. Analysis of the specific Fab-peptide interactions only partially explains the MN isolate specificity shown by this antibody. |
==About this Structure== | ==About this Structure== | ||
| - | 1GGI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1GGI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GGI OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of a human immunodeficiency virus type 1 neutralizing antibody, 50.1, in complex with its V3 loop peptide antigen., Rini JM, Stanfield RL, Stura EA, Salinas PA, Profy AT, Wilson IA, Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6325-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8327513 8327513] | Crystal structure of a human immunodeficiency virus type 1 neutralizing antibody, 50.1, in complex with its V3 loop peptide antigen., Rini JM, Stanfield RL, Stura EA, Salinas PA, Profy AT, Wilson IA, Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6325-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8327513 8327513] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Rini, J | + | [[Category: Rini, J M.]] |
| - | [[Category: Stanfield, R | + | [[Category: Stanfield, R L.]] |
| - | [[Category: Wilson, I | + | [[Category: Wilson, I A.]] |
[[Category: immunoglobulin]] | [[Category: immunoglobulin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:49:51 2008'' |
Revision as of 10:49, 21 February 2008
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CRYSTAL STRUCTURE OF AN HIV-1 NEUTRALIZING ANTIBODY 50.1 IN COMPLEX WITH ITS V3 LOOP PEPTIDE ANTIGEN
Overview
The crystal structure of the Fab fragment of a human immunodeficiency virus type 1 (HIV-1) neutralizing monoclonal antibody Fab has been determined at 2.8 A resolution in complex with a linear 16-residue peptide from the third hypervariable region (V3) of gp120. The first 9 residues of the peptide are ordered in the electron density maps, and their conformation is in partial agreement with the beta-strand-type II beta-turn structure predicted for this portion of the V3 loop. Notably, several of the peptide residues that are well conserved among different HIV-1 isolates contact a nonpolar 25-A-long groove in the antibody-combining site. The largely extended structure of the peptide differs from the beta-turns seen as the primary determinants in other published anti-peptide Fab structures. Analysis of the specific Fab-peptide interactions only partially explains the MN isolate specificity shown by this antibody.
About this Structure
1GGI is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a human immunodeficiency virus type 1 neutralizing antibody, 50.1, in complex with its V3 loop peptide antigen., Rini JM, Stanfield RL, Stura EA, Salinas PA, Profy AT, Wilson IA, Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6325-9. PMID:8327513
Page seeded by OCA on Thu Feb 21 12:49:51 2008
