1gik
From Proteopedia
(New page: 200px<br /><applet load="1gik" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gik, resolution 1.80Å" /> '''POKEWEED ANTIVIRAL P...) |
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| - | [[Image:1gik.gif|left|200px]]<br /><applet load="1gik" size=" | + | [[Image:1gik.gif|left|200px]]<br /><applet load="1gik" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gik, resolution 1.80Å" /> | caption="1gik, resolution 1.80Å" /> | ||
'''POKEWEED ANTIVIRAL PROTEIN FROM SEEDS'''<br /> | '''POKEWEED ANTIVIRAL PROTEIN FROM SEEDS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of pokeweed antiviral protein from seeds of | + | The crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana (PAP-S) was solved at 1.8A. PAP-S is a one-chain ribosome-inactivating protein (RIP) and distinctively contains three well-defined N-acetylglucosamines, each covalently linked to an asparagine residue at positions, 10, 44, and 255, respectively. The high-resolution structure clearly shows the three mono-sugars to have either an alpha- or a beta-conformation. Two of sugars are located on the same side of the molecule with the active pocket. Except one hydrogen bond, there are no intermolecular interactions between the polypeptide chain and the sugars. Instead the sugar conformations appear to be stabilized by intermolecular interactions. The sugar structure defined at high resolution provides a structural basis for understanding their possible biological activity. The structural comparisons of PAP-S with other PAPs reveal that the major disparity of these homologous molecules is the different charge distribution on the upper right side of the front side near the active pocket. Based on the available structure of the 50S ribosomal subunit, the possible interactions between PAPs and the ribosome are discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1GIK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana] with NAG and NDG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http:// | + | 1GIK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana] with <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=NDG:'>NDG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GIK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: rRNA N-glycosylase]] | [[Category: rRNA N-glycosylase]] | ||
| - | [[Category: He, X | + | [[Category: He, X L.]] |
[[Category: Hu, Z.]] | [[Category: Hu, Z.]] | ||
| - | [[Category: Li, H | + | [[Category: Li, H M.]] |
| - | [[Category: Wang, D | + | [[Category: Wang, D C.]] |
| - | [[Category: Zeng, Z | + | [[Category: Zeng, Z H.]] |
[[Category: NAG]] | [[Category: NAG]] | ||
[[Category: NDG]] | [[Category: NDG]] | ||
[[Category: alpha+beta]] | [[Category: alpha+beta]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:50:28 2008'' |
Revision as of 10:50, 21 February 2008
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POKEWEED ANTIVIRAL PROTEIN FROM SEEDS
Overview
The crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana (PAP-S) was solved at 1.8A. PAP-S is a one-chain ribosome-inactivating protein (RIP) and distinctively contains three well-defined N-acetylglucosamines, each covalently linked to an asparagine residue at positions, 10, 44, and 255, respectively. The high-resolution structure clearly shows the three mono-sugars to have either an alpha- or a beta-conformation. Two of sugars are located on the same side of the molecule with the active pocket. Except one hydrogen bond, there are no intermolecular interactions between the polypeptide chain and the sugars. Instead the sugar conformations appear to be stabilized by intermolecular interactions. The sugar structure defined at high resolution provides a structural basis for understanding their possible biological activity. The structural comparisons of PAP-S with other PAPs reveal that the major disparity of these homologous molecules is the different charge distribution on the upper right side of the front side near the active pocket. Based on the available structure of the 50S ribosomal subunit, the possible interactions between PAPs and the ribosome are discussed.
About this Structure
1GIK is a Single protein structure of sequence from Phytolacca americana with and as ligands. Active as rRNA N-glycosylase, with EC number 3.2.2.22 Full crystallographic information is available from OCA.
Reference
Crystal structure of pokeweed antiviral protein with well-defined sugars from seeds at 1.8A resolution., Zeng ZH, He XL, Li HM, Hu Z, Wang DC, J Struct Biol. 2003 Feb;141(2):171-8. PMID:12615543
Page seeded by OCA on Thu Feb 21 12:50:28 2008
Categories: Phytolacca americana | Single protein | RRNA N-glycosylase | He, X L. | Hu, Z. | Li, H M. | Wang, D C. | Zeng, Z H. | NAG | NDG | Alpha+beta
