1gix
From Proteopedia
(New page: 200px<br /><applet load="1gix" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gix, resolution 5.5Å" /> '''Crystal structure of ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1gix.jpg|left|200px]]<br /><applet load="1gix" size=" | + | [[Image:1gix.jpg|left|200px]]<br /><applet load="1gix" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gix, resolution 5.5Å" /> | caption="1gix, resolution 5.5Å" /> | ||
'''Crystal structure of the ribosome at 5.5 A resolution. This file, 1GIX, contains the 30S ribosome subunit, three tRNA, and mRNA molecules. 50S ribosome subunit is in the file 1GIY'''<br /> | '''Crystal structure of the ribosome at 5.5 A resolution. This file, 1GIX, contains the 30S ribosome subunit, three tRNA, and mRNA molecules. 50S ribosome subunit is in the file 1GIY'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We describe the crystal structure of the complete Thermus thermophilus 70S | + | We describe the crystal structure of the complete Thermus thermophilus 70S ribosome containing bound messenger RNA and transfer RNAs (tRNAs) at 5.5 angstrom resolution. All of the 16S, 23S, and 5S ribosomal RNA (rRNA) chains, the A-, P-, and E-site tRNAs, and most of the ribosomal proteins can be fitted to the electron density map. The core of the interface between the 30S small subunit and the 50S large subunit, where the tRNA substrates are bound, is dominated by RNA, with proteins located mainly at the periphery, consistent with ribosomal function being based on rRNA. In each of the three tRNA binding sites, the ribosome contacts all of the major elements of tRNA, providing an explanation for the conservation of tRNA structure. The tRNAs are closely juxtaposed with the intersubunit bridges, in a way that suggests coupling of the 20 to 50 angstrom movements associated with tRNA translocation with intersubunit movement. |
==About this Structure== | ==About this Structure== | ||
| - | 1GIX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http:// | + | 1GIX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GIX OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Baucom, A.]] | [[Category: Baucom, A.]] | ||
| - | [[Category: Cate, J | + | [[Category: Cate, J H.D.]] |
| - | [[Category: Earnest, T | + | [[Category: Earnest, T N.]] |
[[Category: Lieberman, K.]] | [[Category: Lieberman, K.]] | ||
| - | [[Category: Noller, H | + | [[Category: Noller, H F.]] |
| - | [[Category: Yusupov, M | + | [[Category: Yusupov, M M.]] |
| - | [[Category: Yusupova, G | + | [[Category: Yusupova, G Z.]] |
[[Category: life]] | [[Category: life]] | ||
[[Category: protein synthesis]] | [[Category: protein synthesis]] | ||
[[Category: ribosome assembly]] | [[Category: ribosome assembly]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:50:33 2008'' |
Revision as of 10:50, 21 February 2008
|
Crystal structure of the ribosome at 5.5 A resolution. This file, 1GIX, contains the 30S ribosome subunit, three tRNA, and mRNA molecules. 50S ribosome subunit is in the file 1GIY
Overview
We describe the crystal structure of the complete Thermus thermophilus 70S ribosome containing bound messenger RNA and transfer RNAs (tRNAs) at 5.5 angstrom resolution. All of the 16S, 23S, and 5S ribosomal RNA (rRNA) chains, the A-, P-, and E-site tRNAs, and most of the ribosomal proteins can be fitted to the electron density map. The core of the interface between the 30S small subunit and the 50S large subunit, where the tRNA substrates are bound, is dominated by RNA, with proteins located mainly at the periphery, consistent with ribosomal function being based on rRNA. In each of the three tRNA binding sites, the ribosome contacts all of the major elements of tRNA, providing an explanation for the conservation of tRNA structure. The tRNAs are closely juxtaposed with the intersubunit bridges, in a way that suggests coupling of the 20 to 50 angstrom movements associated with tRNA translocation with intersubunit movement.
About this Structure
1GIX is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the ribosome at 5.5 A resolution., Yusupov MM, Yusupova GZ, Baucom A, Lieberman K, Earnest TN, Cate JH, Noller HF, Science. 2001 May 4;292(5518):883-96. Epub 2001 Mar 29. PMID:11283358
Page seeded by OCA on Thu Feb 21 12:50:33 2008
