1gqu
From Proteopedia
(New page: 200px<br /><applet load="1gqu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gqu, resolution 2.5Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1gqu.gif|left|200px]]<br /><applet load="1gqu" size=" | + | [[Image:1gqu.gif|left|200px]]<br /><applet load="1gqu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gqu, resolution 2.5Å" /> | caption="1gqu, resolution 2.5Å" /> | ||
'''CRYSTAL STRUCTURE OF AN ALTERNATING A-T OLIGONUCLEOTIDE FRAGMENT WITH HOOGSTEEN BASE PAIRING'''<br /> | '''CRYSTAL STRUCTURE OF AN ALTERNATING A-T OLIGONUCLEOTIDE FRAGMENT WITH HOOGSTEEN BASE PAIRING'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We report here an alternative double-helical structure of the DNA | + | We report here an alternative double-helical structure of the DNA molecule. It has been found in the d(ATA(Br)UAT) and d(ATATAT) sequences by single-crystal x-ray crystallography. This sequence is found not only in TATA boxes, but also in other regulatory regions of DNA. Bases of the two antiparallel strands form Hoogsteen pairs, with adenines in the syn conformation. The structure is related neither to those found in triple helices nor to parallel DNA duplexes. Its conformational parameters are very similar to those of duplex DNA in the B form. Both forms may coexist under physiological conditions, although the Hoogsteen pairing greatly influences the recognition sites on DNA. Our results demonstrate that an alternative to the classical B-DNA double helix is possible. |
==About this Structure== | ==About this Structure== | ||
| - | 1GQU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1GQU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GQU OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of an antiparallel DNA fragment with Hoogsteen base pairing., Abrescia NG, Thompson A, Huynh-Dinh T, Subirana JA, Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2806-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11880632 11880632] | Crystal structure of an antiparallel DNA fragment with Hoogsteen base pairing., Abrescia NG, Thompson A, Huynh-Dinh T, Subirana JA, Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2806-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11880632 11880632] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Abrescia, N | + | [[Category: Abrescia, N G.A.]] |
[[Category: Huynh-Dinh, T.]] | [[Category: Huynh-Dinh, T.]] | ||
| - | [[Category: Subirana, J | + | [[Category: Subirana, J A.]] |
[[Category: Thompson, A.]] | [[Category: Thompson, A.]] | ||
[[Category: hoogsteen base pairing]] | [[Category: hoogsteen base pairing]] | ||
[[Category: new dna structure]] | [[Category: new dna structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:53:01 2008'' |
Revision as of 10:53, 21 February 2008
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CRYSTAL STRUCTURE OF AN ALTERNATING A-T OLIGONUCLEOTIDE FRAGMENT WITH HOOGSTEEN BASE PAIRING
Overview
We report here an alternative double-helical structure of the DNA molecule. It has been found in the d(ATA(Br)UAT) and d(ATATAT) sequences by single-crystal x-ray crystallography. This sequence is found not only in TATA boxes, but also in other regulatory regions of DNA. Bases of the two antiparallel strands form Hoogsteen pairs, with adenines in the syn conformation. The structure is related neither to those found in triple helices nor to parallel DNA duplexes. Its conformational parameters are very similar to those of duplex DNA in the B form. Both forms may coexist under physiological conditions, although the Hoogsteen pairing greatly influences the recognition sites on DNA. Our results demonstrate that an alternative to the classical B-DNA double helix is possible.
About this Structure
1GQU is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of an antiparallel DNA fragment with Hoogsteen base pairing., Abrescia NG, Thompson A, Huynh-Dinh T, Subirana JA, Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2806-11. PMID:11880632
Page seeded by OCA on Thu Feb 21 12:53:01 2008
