1gyh
From Proteopedia
(New page: 200px<br /><applet load="1gyh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gyh, resolution 1.89Å" /> '''STRUCTURE OF D158A C...) |
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| - | [[Image:1gyh.jpg|left|200px]]<br /><applet load="1gyh" size=" | + | [[Image:1gyh.jpg|left|200px]]<br /><applet load="1gyh" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gyh, resolution 1.89Å" /> | caption="1gyh, resolution 1.89Å" /> | ||
'''STRUCTURE OF D158A CELLVIBRIO CELLULOSA ALPHA-L-ARABINANASE MUTANT'''<br /> | '''STRUCTURE OF D158A CELLVIBRIO CELLULOSA ALPHA-L-ARABINANASE MUTANT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cellvibrio japonicus arabinanase Arb43A hydrolyzes the alpha-1,5-linked | + | Cellvibrio japonicus arabinanase Arb43A hydrolyzes the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The three-dimensional structure of Arb43A, determined at 1.9 A resolution, reveals a five-bladed beta-propeller fold. Arb43A is the first enzyme known to display this topology. A long V-shaped surface groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. Three carboxylates deep in the active site groove provide the general acid and base components for glycosidic bond hydrolysis with inversion of anomeric configuration. |
==About this Structure== | ==About this Structure== | ||
| - | 1GYH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Arabinan_endo-1,5-alpha-L-arabinosidase Arabinan endo-1,5-alpha-L-arabinosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.99 3.2.1.99] Full crystallographic information is available from [http:// | + | 1GYH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Arabinan_endo-1,5-alpha-L-arabinosidase Arabinan endo-1,5-alpha-L-arabinosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.99 3.2.1.99] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GYH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cellvibrio japonicus]] | [[Category: Cellvibrio japonicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Charnock, S | + | [[Category: Charnock, S J.]] |
| - | [[Category: Davies, G | + | [[Category: Davies, G J.]] |
| - | [[Category: Dodson, E | + | [[Category: Dodson, E J.]] |
| - | [[Category: Gilbert, H | + | [[Category: Gilbert, H J.]] |
| - | [[Category: Mckie, V | + | [[Category: Mckie, V A.]] |
[[Category: Nurizzo, D.]] | [[Category: Nurizzo, D.]] | ||
| - | [[Category: Roberts, S | + | [[Category: Roberts, S M.]] |
| - | [[Category: Taylor, E | + | [[Category: Taylor, E J.]] |
| - | [[Category: Turkenburg, J | + | [[Category: Turkenburg, J P.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: arabinanase]] | [[Category: arabinanase]] | ||
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[[Category: pseudomonas]] | [[Category: pseudomonas]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:55:22 2008'' |
Revision as of 10:55, 21 February 2008
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STRUCTURE OF D158A CELLVIBRIO CELLULOSA ALPHA-L-ARABINANASE MUTANT
Overview
Cellvibrio japonicus arabinanase Arb43A hydrolyzes the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The three-dimensional structure of Arb43A, determined at 1.9 A resolution, reveals a five-bladed beta-propeller fold. Arb43A is the first enzyme known to display this topology. A long V-shaped surface groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. Three carboxylates deep in the active site groove provide the general acid and base components for glycosidic bond hydrolysis with inversion of anomeric configuration.
About this Structure
1GYH is a Single protein structure of sequence from Cellvibrio japonicus with as ligand. Active as Arabinan endo-1,5-alpha-L-arabinosidase, with EC number 3.2.1.99 Full crystallographic information is available from OCA.
Reference
Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold., Nurizzo D, Turkenburg JP, Charnock SJ, Roberts SM, Dodson EJ, McKie VA, Taylor EJ, Gilbert HJ, Davies GJ, Nat Struct Biol. 2002 Sep;9(9):665-8. PMID:12198486
Page seeded by OCA on Thu Feb 21 12:55:22 2008
Categories: Arabinan endo-1,5-alpha-L-arabinosidase | Cellvibrio japonicus | Single protein | Charnock, S J. | Davies, G J. | Dodson, E J. | Gilbert, H J. | Mckie, V A. | Nurizzo, D. | Roberts, S M. | Taylor, E J. | Turkenburg, J P. | CL | Arabinanase | Catalysis | Cellvibrio | Hydrolase | Propeller | Pseudomonas
