1gz0
From Proteopedia
(New page: 200px<br /><applet load="1gz0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gz0, resolution 2.50Å" /> '''23S RIBOSOMAL RNA G2...) |
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| - | [[Image:1gz0.jpg|left|200px]]<br /><applet load="1gz0" size=" | + | [[Image:1gz0.jpg|left|200px]]<br /><applet load="1gz0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gz0, resolution 2.50Å" /> | caption="1gz0, resolution 2.50Å" /> | ||
'''23S RIBOSOMAL RNA G2251 2'O-METHYLTRANSFERASE RLMB'''<br /> | '''23S RIBOSOMAL RNA G2251 2'O-METHYLTRANSFERASE RLMB'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In Escherichia coli, RlmB catalyzes the methylation of guanosine 2251, a | + | In Escherichia coli, RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. The crystal structure of this 2'O-methyltransferase has been determined at 2.5 A resolution. RlmB consists of an N-terminal domain connected by a flexible extended linker to a catalytic C-terminal domain and forms a dimer in solution. The C-terminal domain displays a divergent methyltransferase fold with a unique knotted region, and lacks the classic AdoMet binding site features. The N-terminal domain is similar to ribosomal proteins L7 and L30, suggesting a role in 23S rRNA recognition. The conserved residues in this novel family of 2'O-methyltransferases cluster in the knotted region, suggesting the location of the catalytic and AdoMet binding sites. |
==About this Structure== | ==About this Structure== | ||
| - | 1GZ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1GZ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZ0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: BSGI, Montreal-Kingston | + | [[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]] |
[[Category: Cygler, M.]] | [[Category: Cygler, M.]] | ||
[[Category: Michel, G.]] | [[Category: Michel, G.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:55:36 2008'' |
Revision as of 10:55, 21 February 2008
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23S RIBOSOMAL RNA G2251 2'O-METHYLTRANSFERASE RLMB
Overview
In Escherichia coli, RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. The crystal structure of this 2'O-methyltransferase has been determined at 2.5 A resolution. RlmB consists of an N-terminal domain connected by a flexible extended linker to a catalytic C-terminal domain and forms a dimer in solution. The C-terminal domain displays a divergent methyltransferase fold with a unique knotted region, and lacks the classic AdoMet binding site features. The N-terminal domain is similar to ribosomal proteins L7 and L30, suggesting a role in 23S rRNA recognition. The conserved residues in this novel family of 2'O-methyltransferases cluster in the knotted region, suggesting the location of the catalytic and AdoMet binding sites.
About this Structure
1GZ0 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The structure of the RlmB 23S rRNA methyltransferase reveals a new methyltransferase fold with a unique knot., Michel G, Sauve V, Larocque R, Li Y, Matte A, Cygler M, Structure. 2002 Oct;10(10):1303-15. PMID:12377117
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