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1h1l
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The x-ray crystal structure of NifV(-) Klebsiella pneumoniae nitrogenase | + | The x-ray crystal structure of NifV(-) Klebsiella pneumoniae nitrogenase MoFe protein (NifV(-) Kp1) has been determined and refined to a resolution of 1.9 A. This is the first structure for a nitrogenase MoFe protein with an altered cofactor. Moreover, it is the first direct evidence that the organic acid citrate is not just present, but replaces homocitrate as a ligand to the molybdenum atom of the iron molybdenum cofactor (FeMoco). Subsequent refinement of the structure revealed that the citrate was present at reduced occupancy. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Nitrogenase]] | [[Category: Nitrogenase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Gormal, C | + | [[Category: Gormal, C A.]] |
| - | [[Category: Lawson, D | + | [[Category: Lawson, D M.]] |
| - | [[Category: Mayer, S | + | [[Category: Mayer, S M.]] |
| - | [[Category: Smith, B | + | [[Category: Smith, B E.]] |
[[Category: CFM]] | [[Category: CFM]] | ||
[[Category: CIT]] | [[Category: CIT]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:56:22 2008'' |
Revision as of 10:56, 21 February 2008
|
NITROGENASE MO-FE PROTEIN FROM KLEBSIELLA PNEUMONIAE, NIFV MUTANT
Overview
The x-ray crystal structure of NifV(-) Klebsiella pneumoniae nitrogenase MoFe protein (NifV(-) Kp1) has been determined and refined to a resolution of 1.9 A. This is the first structure for a nitrogenase MoFe protein with an altered cofactor. Moreover, it is the first direct evidence that the organic acid citrate is not just present, but replaces homocitrate as a ligand to the molybdenum atom of the iron molybdenum cofactor (FeMoco). Subsequent refinement of the structure revealed that the citrate was present at reduced occupancy.
About this Structure
1H1L is a Protein complex structure of sequences from Klebsiella pneumoniae with , , , and as ligands. Active as Nitrogenase, with EC number 1.18.6.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystallographic analysis of the MoFe protein of nitrogenase from a nifV mutant of Klebsiella pneumoniae identifies citrate as a ligand to the molybdenum of iron molybdenum cofactor (FeMoco)., Mayer SM, Gormal CA, Smith BE, Lawson DM, J Biol Chem. 2002 Sep 20;277(38):35263-6. Epub 2002 Jul 19. PMID:12133839
Page seeded by OCA on Thu Feb 21 12:56:22 2008
