1h34
From Proteopedia
(New page: 200px<br /><applet load="1h34" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h34, resolution 2.04Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1h34.jpg|left|200px]]<br /><applet load="1h34" size=" | + | [[Image:1h34.jpg|left|200px]]<br /><applet load="1h34" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1h34, resolution 2.04Å" /> | caption="1h34, resolution 2.04Å" /> | ||
'''CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR'''<br /> | '''CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR'''<br /> | ||
==Overview== | ==Overview== | ||
| - | SAD (single-wavelength anomalous diffraction) has enormous potential for | + | SAD (single-wavelength anomalous diffraction) has enormous potential for phasing proteins using only the anomalous signal of the almost ubiquitous native sulfur, but requires extremely precise data. The previously unknown structure of the lima bean trypsin inhibitor (LBTI) was solved using highly redundant data collected to 3 A using a CCD detector with a rotating-anode generator and three-circle goniometer. The seven 'super-S' atoms (disulfide bridges) were located by dual-space recycling with SHELXD and the high solvent content enabled the density-modification program SHELXE to generate high-quality maps despite the modest resolution. Subsequently, a 2.05 A synchrotron data set was collected and used for further phase extension and structure refinement. |
==About this Structure== | ==About this Structure== | ||
| - | 1H34 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phaseolus_lunatus Phaseolus lunatus]. Full crystallographic information is available from [http:// | + | 1H34 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phaseolus_lunatus Phaseolus lunatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H34 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bunkoczi, G.]] | [[Category: Bunkoczi, G.]] | ||
| - | [[Category: Debreczeni, J | + | [[Category: Debreczeni, J E.]] |
[[Category: Girmann, B.]] | [[Category: Girmann, B.]] | ||
| - | [[Category: Sheldrick, G | + | [[Category: Sheldrick, G M.]] |
[[Category: bowman-birk-type proteinase inhibitor]] | [[Category: bowman-birk-type proteinase inhibitor]] | ||
[[Category: serine protease inhibitor]] | [[Category: serine protease inhibitor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:56:51 2008'' |
Revision as of 10:56, 21 February 2008
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CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR
Overview
SAD (single-wavelength anomalous diffraction) has enormous potential for phasing proteins using only the anomalous signal of the almost ubiquitous native sulfur, but requires extremely precise data. The previously unknown structure of the lima bean trypsin inhibitor (LBTI) was solved using highly redundant data collected to 3 A using a CCD detector with a rotating-anode generator and three-circle goniometer. The seven 'super-S' atoms (disulfide bridges) were located by dual-space recycling with SHELXD and the high solvent content enabled the density-modification program SHELXE to generate high-quality maps despite the modest resolution. Subsequently, a 2.05 A synchrotron data set was collected and used for further phase extension and structure refinement.
About this Structure
1H34 is a Single protein structure of sequence from Phaseolus lunatus. Full crystallographic information is available from OCA.
Reference
In-house phase determination of the lima bean trypsin inhibitor: a low-resolution sulfur-SAD case., Debreczeni JE, Bunkoczi G, Girmann B, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):393-5. Epub 2003, Jan 23. PMID:12554963
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