1h5w
From Proteopedia
(New page: 200px<br /><applet load="1h5w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h5w, resolution 2.1Å" /> '''2.1A BACTERIOPHAGE PH...) |
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| - | [[Image:1h5w.jpg|left|200px]]<br /><applet load="1h5w" size=" | + | [[Image:1h5w.jpg|left|200px]]<br /><applet load="1h5w" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1h5w, resolution 2.1Å" /> | caption="1h5w, resolution 2.1Å" /> | ||
'''2.1A BACTERIOPHAGE PHI-29 CONNECTOR'''<br /> | '''2.1A BACTERIOPHAGE PHI-29 CONNECTOR'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional crystal structure of the bacteriophage phi29 | + | The three-dimensional crystal structure of the bacteriophage phi29 connector has been solved and refined to 2.1A resolution. This 422 kDa oligomeric protein connects the head of the phage to its tail and translocates the DNA into the prohead during packaging. Each monomer has an elongated shape and is composed of a central, mainly alpha-helical domain that includes a three-helix bundle, a distal alpha/beta domain and a proximal six-stranded SH3-like domain. The protomers assemble into a 12-mer, propeller-like, super-structure with a 35 A wide central channel. The surface of the channel is mainly electronegative, but it includes two lysine rings 20 A apart. On the external surface of the particle a hydrophobic belt extends to the concave area below the SH3-like domain, which forms a crown that retains the particle in the head. The lipophilic belt contacts the non-matching symmetry vertex of the capsid and forms a bearing for the connector rotation. The structure suggests a translocation mechanism in which the longitudinal displacement of the DNA along its axis is coupled to connector spinning. |
==About this Structure== | ==About this Structure== | ||
| - | 1H5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_phage_f237 Vibrio phage f237] with MPD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1H5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_phage_f237 Vibrio phage f237] with <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H5W OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vibrio phage f237]] | [[Category: Vibrio phage f237]] | ||
| - | [[Category: Carrascosa, J | + | [[Category: Carrascosa, J L.]] |
[[Category: Coll, M.]] | [[Category: Coll, M.]] | ||
| - | [[Category: Gomis-Ruth, F | + | [[Category: Gomis-Ruth, F X.]] |
[[Category: Guasch, A.]] | [[Category: Guasch, A.]] | ||
[[Category: Ibarra, B.]] | [[Category: Ibarra, B.]] | ||
[[Category: Pous, J.]] | [[Category: Pous, J.]] | ||
[[Category: Sousa, N.]] | [[Category: Sousa, N.]] | ||
| - | [[Category: Valpuesta, J | + | [[Category: Valpuesta, J M.]] |
[[Category: MPD]] | [[Category: MPD]] | ||
[[Category: connector]] | [[Category: connector]] | ||
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[[Category: virus]] | [[Category: virus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:57:45 2008'' |
Revision as of 10:57, 21 February 2008
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2.1A BACTERIOPHAGE PHI-29 CONNECTOR
Overview
The three-dimensional crystal structure of the bacteriophage phi29 connector has been solved and refined to 2.1A resolution. This 422 kDa oligomeric protein connects the head of the phage to its tail and translocates the DNA into the prohead during packaging. Each monomer has an elongated shape and is composed of a central, mainly alpha-helical domain that includes a three-helix bundle, a distal alpha/beta domain and a proximal six-stranded SH3-like domain. The protomers assemble into a 12-mer, propeller-like, super-structure with a 35 A wide central channel. The surface of the channel is mainly electronegative, but it includes two lysine rings 20 A apart. On the external surface of the particle a hydrophobic belt extends to the concave area below the SH3-like domain, which forms a crown that retains the particle in the head. The lipophilic belt contacts the non-matching symmetry vertex of the capsid and forms a bearing for the connector rotation. The structure suggests a translocation mechanism in which the longitudinal displacement of the DNA along its axis is coupled to connector spinning.
About this Structure
1H5W is a Single protein structure of sequence from Vibrio phage f237 with as ligand. Full crystallographic information is available from OCA.
Reference
Detailed architecture of a DNA translocating machine: the high-resolution structure of the bacteriophage phi29 connector particle., Guasch A, Pous J, Ibarra B, Gomis-Ruth FX, Valpuesta JM, Sousa N, Carrascosa JL, Coll M, J Mol Biol. 2002 Jan 25;315(4):663-76. PMID:11812138
Page seeded by OCA on Thu Feb 21 12:57:45 2008
Categories: Single protein | Vibrio phage f237 | Carrascosa, J L. | Coll, M. | Gomis-Ruth, F X. | Guasch, A. | Ibarra, B. | Pous, J. | Sousa, N. | Valpuesta, J M. | MPD | Connector | Helix bundle | Portal | Sh3-like | Virus
