1h6j
From Proteopedia
(New page: 200px<br /><applet load="1h6j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h6j, resolution 2.32Å" /> '''THE THREE-DIMENSIONA...) |
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| - | [[Image:1h6j.gif|left|200px]]<br /><applet load="1h6j" size=" | + | [[Image:1h6j.gif|left|200px]]<br /><applet load="1h6j" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1h6j, resolution 2.32Å" /> | caption="1h6j, resolution 2.32Å" /> | ||
'''THE THREE-DIMENSIONAL STRUCTURE OF CAPSULE-SPECIFIC CMP:2-KETO-3-DEOXY-MANNO-OCTONIC ACID SYNTHETASE FROM ESCHERICHIA COLI'''<br /> | '''THE THREE-DIMENSIONAL STRUCTURE OF CAPSULE-SPECIFIC CMP:2-KETO-3-DEOXY-MANNO-OCTONIC ACID SYNTHETASE FROM ESCHERICHIA COLI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | CMP-Kdo synthetases from Gram-negative bacteria activate Kdo for | + | CMP-Kdo synthetases from Gram-negative bacteria activate Kdo for incorporation into lipo- and capsule-polysaccharides. Here we report the crystal structure of the capsule-specific synthetase from E. coli at 2.3 A resolution. The enzyme is a dimer of 2 x 245 amino acid residues assuming C2 symmetry. It contains a central predominantly parallel beta-sheet with surrounding helices. The chain fold is novel; it is remotely related to a double Rossmann fold. A large pocket at the carboxyl terminal ends of the central. beta-strands most likely accommodates the catalytic center. A putative phosphate binding site at the loop between the first beta-strand and the following helix is indicated by a bound iridium hexachloride anion. |
==About this Structure== | ==About this Structure== | ||
| - | 1H6J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/3-deoxy-manno-octulosonate_cytidylyltransferase 3-deoxy-manno-octulosonate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.38 2.7.7.38] Full crystallographic information is available from [http:// | + | 1H6J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/3-deoxy-manno-octulosonate_cytidylyltransferase 3-deoxy-manno-octulosonate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.38 2.7.7.38] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H6J OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Jann, K.]] | [[Category: Jann, K.]] | ||
[[Category: Jelakovic, S.]] | [[Category: Jelakovic, S.]] | ||
| - | [[Category: Schulz, G | + | [[Category: Schulz, G E.]] |
[[Category: capsular polysaccharide]] | [[Category: capsular polysaccharide]] | ||
[[Category: cmp-kdo synthetase]] | [[Category: cmp-kdo synthetase]] | ||
[[Category: saccharide activation]] | [[Category: saccharide activation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:57:57 2008'' |
Revision as of 10:58, 21 February 2008
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THE THREE-DIMENSIONAL STRUCTURE OF CAPSULE-SPECIFIC CMP:2-KETO-3-DEOXY-MANNO-OCTONIC ACID SYNTHETASE FROM ESCHERICHIA COLI
Overview
CMP-Kdo synthetases from Gram-negative bacteria activate Kdo for incorporation into lipo- and capsule-polysaccharides. Here we report the crystal structure of the capsule-specific synthetase from E. coli at 2.3 A resolution. The enzyme is a dimer of 2 x 245 amino acid residues assuming C2 symmetry. It contains a central predominantly parallel beta-sheet with surrounding helices. The chain fold is novel; it is remotely related to a double Rossmann fold. A large pocket at the carboxyl terminal ends of the central. beta-strands most likely accommodates the catalytic center. A putative phosphate binding site at the loop between the first beta-strand and the following helix is indicated by a bound iridium hexachloride anion.
About this Structure
1H6J is a Single protein structure of sequence from Escherichia coli. Active as 3-deoxy-manno-octulosonate cytidylyltransferase, with EC number 2.7.7.38 Full crystallographic information is available from OCA.
Reference
The three-dimensional structure of capsule-specific CMP: 2-keto-3-deoxy-manno-octonic acid synthetase from Escherichia coli., Jelakovic S, Jann K, Schulz GE, FEBS Lett. 1996 Aug 5;391(1-2):157-61. PMID:8706906
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