1hb8
From Proteopedia
(New page: 200px<br /><applet load="1hb8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hb8, resolution 2.00Å" /> '''STRUCTURE OF BOVINE ...) |
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| - | [[Image:1hb8.jpg|left|200px]]<br /><applet load="1hb8" size=" | + | [[Image:1hb8.jpg|left|200px]]<br /><applet load="1hb8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1hb8, resolution 2.00Å" /> | caption="1hb8, resolution 2.00Å" /> | ||
'''STRUCTURE OF BOVINE ACYL-COA BINDING PROTEIN IN TETRAGONAL CRYSTAL FORM'''<br /> | '''STRUCTURE OF BOVINE ACYL-COA BINDING PROTEIN IN TETRAGONAL CRYSTAL FORM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Acyl-CoA binding protein (ACBP) maintains a pool of fatty acyl-CoA | + | Acyl-CoA binding protein (ACBP) maintains a pool of fatty acyl-CoA molecules in the cell and plays a role in fatty acid metabolism. The biochemical properties of Plasmodium falciparum ACBP are described together with the 2.0 A resolution crystal structures of a P. falciparum ACBP-acyl-CoA complex and of bovine ACBP in two crystal forms. Overall, the bovine ACBP crystal structures are similar to the NMR structures published previously; however, the bovine and parasite ACBP structures are less similar. The parasite ACBP is shown to have a different ligand-binding pocket, leading to an acyl-CoA binding specificity different from that of bovine ACBP. Several non-conservative differences in residues that interact with the ligand were identified between the mammalian and parasite ACBPs. These, together with measured binding-specificity differences, suggest that there is a potential for the design of molecules that might selectively block the acyl-CoA binding site. |
==About this Structure== | ==About this Structure== | ||
| - | 1HB8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1HB8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HB8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bergfors, T.]] | [[Category: Bergfors, T.]] | ||
| - | [[Category: Jones, T | + | [[Category: Jones, T A.]] |
| - | [[Category: Kleywegt, G | + | [[Category: Kleywegt, G J.]] |
[[Category: Knudsen, J.]] | [[Category: Knudsen, J.]] | ||
| - | [[Category: Zou, J | + | [[Category: Zou, J Y.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: acyl-coa]] | [[Category: acyl-coa]] | ||
[[Category: binding protein]] | [[Category: binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:59:25 2008'' |
Revision as of 10:59, 21 February 2008
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STRUCTURE OF BOVINE ACYL-COA BINDING PROTEIN IN TETRAGONAL CRYSTAL FORM
Overview
Acyl-CoA binding protein (ACBP) maintains a pool of fatty acyl-CoA molecules in the cell and plays a role in fatty acid metabolism. The biochemical properties of Plasmodium falciparum ACBP are described together with the 2.0 A resolution crystal structures of a P. falciparum ACBP-acyl-CoA complex and of bovine ACBP in two crystal forms. Overall, the bovine ACBP crystal structures are similar to the NMR structures published previously; however, the bovine and parasite ACBP structures are less similar. The parasite ACBP is shown to have a different ligand-binding pocket, leading to an acyl-CoA binding specificity different from that of bovine ACBP. Several non-conservative differences in residues that interact with the ligand were identified between the mammalian and parasite ACBPs. These, together with measured binding-specificity differences, suggest that there is a potential for the design of molecules that might selectively block the acyl-CoA binding site.
About this Structure
1HB8 is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Binding site differences revealed by crystal structures of Plasmodium falciparum and bovine acyl-CoA binding protein., van Aalten DM, Milne KG, Zou JY, Kleywegt GJ, Bergfors T, Ferguson MA, Knudsen J, Jones TA, J Mol Biol. 2001 May 25;309(1):181-92. PMID:11491287
Page seeded by OCA on Thu Feb 21 12:59:25 2008
