1hbz
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The three-dimensional crystal structure of catalase from Micrococcus | + | The three-dimensional crystal structure of catalase from Micrococcus lysodeikticus has been solved by multiple isomorphous replacement and refined at 1.5 A resolution. The subunit of the tetrameric molecule of 222 symmetry consists of a single polypeptide chain of about 500 amino acid residues and one haem group. The crystals belong to space group P4(2)2(1)2 with unit cell parameters a = b = 106.7 A, c = 106.3 A, and there is one subunit of the tetramer per asymmetric unit. The amino acid sequence has been tentatively determined by computer graphics model building and comparison with the known three-dimensional structure of beef liver catalase and sequences of several other catalases. The atomic model has been refined by Hendrickson and Konnert's least-squares minimisation against 94,315 reflections between 8 A and 1.5 A. The final model consists of 3,977 non-hydrogen atoms of the protein and haem group, 426 water molecules and one sulphate ion. The secondary and tertiary structures of the bacterial catalase have been analyzed and a comparison with the structure of beef liver catalase has been made. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Micrococcus luteus]] | [[Category: Micrococcus luteus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Barynin, V | + | [[Category: Barynin, V V.]] |
[[Category: Braningen, J.]] | [[Category: Braningen, J.]] | ||
[[Category: Dauter, Z.]] | [[Category: Dauter, Z.]] | ||
| - | [[Category: Grebenko, A | + | [[Category: Grebenko, A I.]] |
| - | [[Category: Melik-Adamyan, W | + | [[Category: Melik-Adamyan, W R.]] |
| - | [[Category: Murshudov, G | + | [[Category: Murshudov, G N.]] |
| - | [[Category: Wilson, K | + | [[Category: Wilson, K S.]] |
[[Category: HEM]] | [[Category: HEM]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: peroxidase]] | [[Category: peroxidase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:59:37 2008'' |
Revision as of 10:59, 21 February 2008
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CATALASE FROM MICROCOCCUS LYSODEIKTICU
Overview
The three-dimensional crystal structure of catalase from Micrococcus lysodeikticus has been solved by multiple isomorphous replacement and refined at 1.5 A resolution. The subunit of the tetrameric molecule of 222 symmetry consists of a single polypeptide chain of about 500 amino acid residues and one haem group. The crystals belong to space group P4(2)2(1)2 with unit cell parameters a = b = 106.7 A, c = 106.3 A, and there is one subunit of the tetramer per asymmetric unit. The amino acid sequence has been tentatively determined by computer graphics model building and comparison with the known three-dimensional structure of beef liver catalase and sequences of several other catalases. The atomic model has been refined by Hendrickson and Konnert's least-squares minimisation against 94,315 reflections between 8 A and 1.5 A. The final model consists of 3,977 non-hydrogen atoms of the protein and haem group, 426 water molecules and one sulphate ion. The secondary and tertiary structures of the bacterial catalase have been analyzed and a comparison with the structure of beef liver catalase has been made.
About this Structure
1HBZ is a Single protein structure of sequence from Micrococcus luteus with and as ligands. Active as Catalase, with EC number 1.11.1.6 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of catalase from Micrococcus lysodeikticus at 1.5 A resolution., Murshudov GN, Melik-Adamyan WR, Grebenko AI, Barynin VV, Vagin AA, Vainshtein BK, Dauter Z, Wilson KS, FEBS Lett. 1992 Nov 9;312(2-3):127-31. PMID:1426241
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