7a3h
From Proteopedia
(New page: 200px<br /> <applet load="7a3h" size="450" color="white" frame="true" align="right" spinBox="true" caption="7a3h, resolution 0.95Å" /> '''NATIVE ENDOGLUCANAS...) |
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==About this Structure== | ==About this Structure== | ||
| - | 7A3H is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_agaradhaerens Bacillus agaradhaerens]] with GOL and EOH as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=7A3H OCA]]. | + | 7A3H is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_agaradhaerens Bacillus agaradhaerens]] with GOL and EOH as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Cellulase Cellulase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Structure known Active Sites: ACI and NUC. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=7A3H OCA]]. |
==Reference== | ==Reference== | ||
Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase., Davies GJ, Mackenzie L, Varrot A, Dauter M, Brzozowski AM, Schulein M, Withers SG, Biochemistry. 1998 Aug 25;37(34):11707-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9718293 9718293] | Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase., Davies GJ, Mackenzie L, Varrot A, Dauter M, Brzozowski AM, Schulein M, Withers SG, Biochemistry. 1998 Aug 25;37(34):11707-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9718293 9718293] | ||
[[Category: Bacillus agaradhaerens]] | [[Category: Bacillus agaradhaerens]] | ||
| + | [[Category: Cellulase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Brzozowski, A.M.]] | [[Category: Brzozowski, A.M.]] | ||
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[[Category: skew-boat]] | [[Category: skew-boat]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:13:54 2007'' |
Revision as of 12:09, 30 October 2007
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NATIVE ENDOGLUCANASE CEL5A CATALYTIC CORE DOMAIN AT 0.95 ANGSTROMS RESOLUTION
Overview
The enzymatic hydrolysis of O-glycosidic linkages is one of the most, diverse and widespread reactions in nature and involves a classic, "textbook" enzyme mechanism. A multidisciplinary analysis of a, beta-glycoside hydrolase, the Cel5A from Bacillus agaradhaerens, is, presented in which the structures of each of the native, substrate, covalent-intermediate, and product complexes have been determined and, their interconversions analyzed kinetically, providing unprecedented, insights into the mechanism of this enzyme class. Substrate is bound in a, distorted 1S3 skew-boat conformation, thereby presenting the anomeric, carbon appropriately for nucleophilic attack as well as satisfying the, stereoelectronic requirements for an incipient oxocarbenium ion. Leaving, group departure results in ... [(full description)]
About this Structure
7A3H is a [Single protein] structure of sequence from [Bacillus agaradhaerens] with GOL and EOH as [ligands]. Active as [Cellulase], with EC number [3.2.1.4]. Structure known Active Sites: ACI and NUC. Full crystallographic information is available from [OCA].
Reference
Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase., Davies GJ, Mackenzie L, Varrot A, Dauter M, Brzozowski AM, Schulein M, Withers SG, Biochemistry. 1998 Aug 25;37(34):11707-13. PMID:9718293
Page seeded by OCA on Tue Oct 30 14:13:54 2007
Categories: Bacillus agaradhaerens | Cellulase | Single protein | Brzozowski, A.M. | Dauter, M. | Davies, G.J. | Mackenzie, L. | Schulein, M. | Varrot, A. | Withers, S.G. | EOH | GOL | Cellulose degradation | Distortion | Endoglucanase | Glycoside hydrolase family 5 | Hydrolase | Michaelis complex | Skew-boat
