1hbx
From Proteopedia
(New page: 200px<br /> <applet load="1hbx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hbx, resolution 3.15Å" /> '''TERNARY COMPLEX OF ...) |
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| - | [[Image:1hbx.gif|left|200px]]<br /> | + | [[Image:1hbx.gif|left|200px]]<br /><applet load="1hbx" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1hbx, resolution 3.15Å" /> | caption="1hbx, resolution 3.15Å" /> | ||
'''TERNARY COMPLEX OF SAP-1 AND SRF WITH SPECIFIC SRE DNA'''<br /> | '''TERNARY COMPLEX OF SAP-1 AND SRF WITH SPECIFIC SRE DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The serum response element (SRE) is found in several immediate-early gene | + | The serum response element (SRE) is found in several immediate-early gene promoters. This DNA sequence is necessary and sufficient for rapid transcriptional induction of the human c-fos proto-oncogene in response to stimuli external to the cell. Full activation of the SRE requires the cooperative binding of a ternary complex factor (TCF) and serum response factor (SRF) to their specific DNA sites. The X-ray structure of the human SAP-1-SRF-SRE DNA ternary complex was determined (Protein Data Bank code 1hbx). It shows SAP-1 TCF bound to SRF through interactions between the SAP-1 B-box and SRF MADS domain in addition to contacts between their respective DNA-binding motifs. The SAP-1 B-box is part of a flexible linker of which 21 amino acids become ordered upon ternary complex formation. Comparison with a similar region from the yeast MATalpha2-MCM1-DNA complex suggests a common binding motif through which MADS-box proteins may interact with additional factors such as Fli-1. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1HBX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1HBX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HBX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Hassler, M.]] | [[Category: Hassler, M.]] | ||
| - | [[Category: Richmond, T | + | [[Category: Richmond, T J.]] |
[[Category: gene regulation]] | [[Category: gene regulation]] | ||
[[Category: serum response factor]] | [[Category: serum response factor]] | ||
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[[Category: transcription complex]] | [[Category: transcription complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:59:40 2008'' |
Revision as of 10:59, 21 February 2008
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TERNARY COMPLEX OF SAP-1 AND SRF WITH SPECIFIC SRE DNA
Contents |
Overview
The serum response element (SRE) is found in several immediate-early gene promoters. This DNA sequence is necessary and sufficient for rapid transcriptional induction of the human c-fos proto-oncogene in response to stimuli external to the cell. Full activation of the SRE requires the cooperative binding of a ternary complex factor (TCF) and serum response factor (SRF) to their specific DNA sites. The X-ray structure of the human SAP-1-SRF-SRE DNA ternary complex was determined (Protein Data Bank code 1hbx). It shows SAP-1 TCF bound to SRF through interactions between the SAP-1 B-box and SRF MADS domain in addition to contacts between their respective DNA-binding motifs. The SAP-1 B-box is part of a flexible linker of which 21 amino acids become ordered upon ternary complex formation. Comparison with a similar region from the yeast MATalpha2-MCM1-DNA complex suggests a common binding motif through which MADS-box proteins may interact with additional factors such as Fli-1.
Disease
Known disease associated with this structure: Osteoarthritis of hip, female-specific, susceptibility to OMIM:[605083]
About this Structure
1HBX is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The B-box dominates SAP-1-SRF interactions in the structure of the ternary complex., Hassler M, Richmond TJ, EMBO J. 2001 Jun 15;20(12):3018-28. PMID:11406578
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