1hcj
From Proteopedia
(New page: 200px<br /><applet load="1hcj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hcj, resolution 1.80Å" /> '''PHOTOPRODUCT OF THE ...) |
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| - | [[Image:1hcj.jpg|left|200px]]<br /><applet load="1hcj" size=" | + | [[Image:1hcj.jpg|left|200px]]<br /><applet load="1hcj" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1hcj, resolution 1.80Å" /> | caption="1hcj, resolution 1.80Å" /> | ||
'''PHOTOPRODUCT OF THE WILD-TYPE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN'''<br /> | '''PHOTOPRODUCT OF THE WILD-TYPE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs | + | Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP(398)) into one absorbing at 483 nm (GFP(483)). Here we show that this photoconversion of GFP is a one-photon process that is paralleled by decarboxylation of Glu 222. We propose a mechanism in which decarboxylation is due to electron transfer between the gamma-carboxylate of Glu 222 and the p-hydroxybenzylidene-imidazolidinone chromophore of GFP, followed by reverse transfer of an electron and a proton to the remaining carbon side chain atom of Glu 222. Oxidative decarboxylation of a gamma-carboxylate represents a new type of posttranslational modification that may also occur in enzymes with high-potential reaction intermediates. |
==About this Structure== | ==About this Structure== | ||
| - | 1HCJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http:// | + | 1HCJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HCJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Gensch, T.]] | [[Category: Gensch, T.]] | ||
| - | [[Category: Hellingwerf, K | + | [[Category: Hellingwerf, K J.]] |
[[Category: Johnson, L.]] | [[Category: Johnson, L.]] | ||
| - | [[Category: Thor, J | + | [[Category: Thor, J J.Van.]] |
[[Category: beta-barrel]] | [[Category: beta-barrel]] | ||
[[Category: bioluminescence]] | [[Category: bioluminescence]] | ||
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[[Category: luminescence]] | [[Category: luminescence]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:59:48 2008'' |
Revision as of 10:59, 21 February 2008
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PHOTOPRODUCT OF THE WILD-TYPE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN
Overview
Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP(398)) into one absorbing at 483 nm (GFP(483)). Here we show that this photoconversion of GFP is a one-photon process that is paralleled by decarboxylation of Glu 222. We propose a mechanism in which decarboxylation is due to electron transfer between the gamma-carboxylate of Glu 222 and the p-hydroxybenzylidene-imidazolidinone chromophore of GFP, followed by reverse transfer of an electron and a proton to the remaining carbon side chain atom of Glu 222. Oxidative decarboxylation of a gamma-carboxylate represents a new type of posttranslational modification that may also occur in enzymes with high-potential reaction intermediates.
About this Structure
1HCJ is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.
Reference
Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222., van Thor JJ, Gensch T, Hellingwerf KJ, Johnson LN, Nat Struct Biol. 2002 Jan;9(1):37-41. PMID:11740505
Page seeded by OCA on Thu Feb 21 12:59:48 2008
