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1hji
From Proteopedia
(New page: 200px<br /><applet load="1hji" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hji" /> '''BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA ...) |
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| - | [[Image:1hji.gif|left|200px]]<br /><applet load="1hji" size=" | + | [[Image:1hji.gif|left|200px]]<br /><applet load="1hji" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1hji" /> | caption="1hji" /> | ||
'''BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX'''<br /> | '''BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Nun protein from coliphage HK022 binds to phage boxB RNA and functions, in | + | Nun protein from coliphage HK022 binds to phage boxB RNA and functions, in contrast to phage lambda N protein, as a transcriptional terminator. The basic Nun-(10-44) peptide contains the boxB RNA binding arginine rich motif, ARM. The peptide binds boxB RNA and competes with the phage lambda ARM peptide N-(1-36) as indicated by nuclear magnetic resonance (NMR) spectroscopy titrations. In two-dimensional nuclear Overhauser enhancement spectroscopy experiments boxB RNA in complex with Nun-(20-44) exhibits the same pattern of resonances as it does in complex with N peptides containing the ARM, and we could show that Nun-(20-44) forms a bent alpha-helix upon binding to the boxB RNA. The structure of the boxB RNA-bound Nun-(20-44) was determined on the basis of 191 intra- and 30 intermolecular distance restraints. Ser-24 is anchored to the lower RNA stem, and stacking of Tyr-39 and A7 is clearly experimentally indicated. Arg-28 shows numerous contacts to the RNA stem. Leu-22, Ile-30, Trp-33, Ile-37, and Leu-41 form a hydrophobic surface, which could be a recognition site for additional host factors such as NusG. Such a hydrophobic surface area is not present in N-(1-36) bound to boxB RNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1HJI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk022 Enterobacteria phage hk022]. Full crystallographic information is available from [http:// | + | 1HJI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk022 Enterobacteria phage hk022]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: termination]] | [[Category: termination]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:01:57 2008'' |
Revision as of 11:02, 21 February 2008
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BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX
Overview
Nun protein from coliphage HK022 binds to phage boxB RNA and functions, in contrast to phage lambda N protein, as a transcriptional terminator. The basic Nun-(10-44) peptide contains the boxB RNA binding arginine rich motif, ARM. The peptide binds boxB RNA and competes with the phage lambda ARM peptide N-(1-36) as indicated by nuclear magnetic resonance (NMR) spectroscopy titrations. In two-dimensional nuclear Overhauser enhancement spectroscopy experiments boxB RNA in complex with Nun-(20-44) exhibits the same pattern of resonances as it does in complex with N peptides containing the ARM, and we could show that Nun-(20-44) forms a bent alpha-helix upon binding to the boxB RNA. The structure of the boxB RNA-bound Nun-(20-44) was determined on the basis of 191 intra- and 30 intermolecular distance restraints. Ser-24 is anchored to the lower RNA stem, and stacking of Tyr-39 and A7 is clearly experimentally indicated. Arg-28 shows numerous contacts to the RNA stem. Leu-22, Ile-30, Trp-33, Ile-37, and Leu-41 form a hydrophobic surface, which could be a recognition site for additional host factors such as NusG. Such a hydrophobic surface area is not present in N-(1-36) bound to boxB RNA.
About this Structure
1HJI is a Protein complex structure of sequences from Enterobacteria phage hk022. Full crystallographic information is available from OCA.
Reference
The structure of the coliphage HK022 Nun protein-lambda-phage boxB RNA complex. Implications for the mechanism of transcription termination., Faber C, Scharpf M, Becker T, Sticht H, Rosch P, J Biol Chem. 2001 Aug 24;276(34):32064-70. Epub 2001 May 16. PMID:11356847
Page seeded by OCA on Thu Feb 21 13:01:57 2008
