1hk8
From Proteopedia
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==Overview== | ==Overview== | ||
| - | A Zn(Cys)(4) center has been found in the C-terminal region of the crystal | + | A Zn(Cys)(4) center has been found in the C-terminal region of the crystal structure of the anaerobic class III ribonucleotide reductase (RNR) from bacteriophage T4. The metal center is structurally related to the zinc ribbon motif and to rubredoxin and rubrerythrin. Mutant enzymes of the homologous RNR from Escherichia coli, in which the coordinating cysteines, conserved in almost all known class III RNR sequences, have been mutated into alanines, are shown to be inactive as the result of their inability to generate the catalytically essential glycyl radical. The possible roles of the metal center are discussed in relationship to the currently proposed reaction mechanism for generation of the glycyl radical in class III RNRs. |
==About this Structure== | ==About this Structure== | ||
| - | 1HK8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t2 Enterobacteria phage t2] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=DGT:'>DGT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure | + | 1HK8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t2 Enterobacteria phage t2] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=DGT:'>DGT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1H77. Active as [http://en.wikipedia.org/wiki/Ribonucleoside-triphosphate_reductase Ribonucleoside-triphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.2 1.17.4.2] Known structural/functional Site: <scene name='pdbsite=DG1:Zn2++Binding+Site+For+Chain+A'>DG1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HK8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Andersson, J.]] | [[Category: Andersson, J.]] | ||
| - | [[Category: Larsson, K | + | [[Category: Larsson, K M.]] |
| - | [[Category: Logan, D | + | [[Category: Logan, D T.]] |
[[Category: Nordlund, P.]] | [[Category: Nordlund, P.]] | ||
| - | [[Category: Sjoeberg, B | + | [[Category: Sjoeberg, B M.]] |
[[Category: DGT]] | [[Category: DGT]] | ||
[[Category: MN]] | [[Category: MN]] | ||
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[[Category: substrate specificity]] | [[Category: substrate specificity]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:02:07 2008'' |
Revision as of 11:02, 21 February 2008
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STRUCTURAL BASIS FOR ALLOSTERIC SUBSTRATE SPECIFICITY REGULATION IN CLASS III RIBONUCLEOTIDE REDUCTASES: NRDD IN COMPLEX WITH DGTP
Overview
A Zn(Cys)(4) center has been found in the C-terminal region of the crystal structure of the anaerobic class III ribonucleotide reductase (RNR) from bacteriophage T4. The metal center is structurally related to the zinc ribbon motif and to rubredoxin and rubrerythrin. Mutant enzymes of the homologous RNR from Escherichia coli, in which the coordinating cysteines, conserved in almost all known class III RNR sequences, have been mutated into alanines, are shown to be inactive as the result of their inability to generate the catalytically essential glycyl radical. The possible roles of the metal center are discussed in relationship to the currently proposed reaction mechanism for generation of the glycyl radical in class III RNRs.
About this Structure
1HK8 is a Single protein structure of sequence from Enterobacteria phage t2 with , and as ligands. This structure supersedes the now removed PDB entry 1H77. Active as Ribonucleoside-triphosphate reductase, with EC number 1.17.4.2 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
A metal-binding site in the catalytic subunit of anaerobic ribonucleotide reductase., Logan DT, Mulliez E, Larsson KM, Bodevin S, Atta M, Garnaud PE, Sjoberg BM, Fontecave M, Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3826-31. Epub 2003 Mar 24. PMID:12655046
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