1hkz
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | The CotA laccase from the endospore coat of Bacillus subtilis has been | + | The CotA laccase from the endospore coat of Bacillus subtilis has been crystallized in the presence of the non-catalytic co-oxidant 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and the structure was determined using synchrotron radiation. The binding site for this adduct is well defined and indicates how ABTS, in conjunction with laccases, could act as an oxidative mediator toward non-phenolic moieties. In addition, a dioxygen moiety is clearly defined within the solvent channel oriented toward one of the T3 copper atoms in the trinuclear center. |
==About this Structure== | ==About this Structure== | ||
| Line 13: | Line 13: | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Carrondo, M | + | [[Category: Carrondo, M A.]] |
| - | [[Category: Enguita, F | + | [[Category: Enguita, F J.]] |
[[Category: Grenha, R.]] | [[Category: Grenha, R.]] | ||
| - | [[Category: Henriques, A | + | [[Category: Henriques, A O.]] |
[[Category: Marcal, D.]] | [[Category: Marcal, D.]] | ||
| - | [[Category: Martins, L | + | [[Category: Martins, L O.]] |
[[Category: C1O]] | [[Category: C1O]] | ||
[[Category: CU]] | [[Category: CU]] | ||
| Line 30: | Line 30: | ||
[[Category: sporulation]] | [[Category: sporulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:02:21 2008'' |
Revision as of 11:02, 21 February 2008
|
CRYSTAL STRUCTURE OF BACILLUS SUBTILIS COTA AFTER 8H SOAKING WITH ABTS
Overview
The CotA laccase from the endospore coat of Bacillus subtilis has been crystallized in the presence of the non-catalytic co-oxidant 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and the structure was determined using synchrotron radiation. The binding site for this adduct is well defined and indicates how ABTS, in conjunction with laccases, could act as an oxidative mediator toward non-phenolic moieties. In addition, a dioxygen moiety is clearly defined within the solvent channel oriented toward one of the T3 copper atoms in the trinuclear center.
About this Structure
1HKZ is a Single protein structure of sequence from Bacillus subtilis with , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Substrate and dioxygen binding to the endospore coat laccase from Bacillus subtilis., Enguita FJ, Marcal D, Martins LO, Grenha R, Henriques AO, Lindley PF, Carrondo MA, J Biol Chem. 2004 May 28;279(22):23472-6. Epub 2004 Feb 4. PMID:14764581
Page seeded by OCA on Thu Feb 21 13:02:21 2008
Categories: Bacillus subtilis | Single protein | Carrondo, M A. | Enguita, F J. | Grenha, R. | Henriques, A O. | Marcal, D. | Martins, L O. | C1O | CU | CU1 | GOL | Copper-binding | Endospore coat | Laccase | Oxidase | Reaction cycle | Sporulation
