1hml
From Proteopedia
(New page: 200px<br /> <applet load="1hml" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hml, resolution 1.7Å" /> '''ALPHA_LACTALBUMIN PO...) |
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| - | [[Image:1hml.gif|left|200px]]<br /> | + | [[Image:1hml.gif|left|200px]]<br /><applet load="1hml" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1hml" size=" | + | |
caption="1hml, resolution 1.7Å" /> | caption="1hml, resolution 1.7Å" /> | ||
'''ALPHA_LACTALBUMIN POSSESSES A DISTINCT ZINC BINDING SITE'''<br /> | '''ALPHA_LACTALBUMIN POSSESSES A DISTINCT ZINC BINDING SITE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | It has been proposed that the binding of Zn2+ to alpha-lactalbumin | + | It has been proposed that the binding of Zn2+ to alpha-lactalbumin switches the conformation to one akin to a state intermediate in the folding of the protein. However, the high resolution x-ray crystal structure of human alpha-lactalbumin-Zn2+ complex at 1.7-A resolution (pH 7.6) does not reveal any significant change in conformation from the native state. The Zn2+ ion binds specifically in the "cleft" of alpha-lactalbumin (the region which forms the active site of the homologous protein lysozyme). This may suggest a possible role for Zn2+ binding in lactose synthase complex. The coordination of the Zn2+ ion involves a symmetry-related molecule in the crystal, the crystal contacts being stabilized by a SO4(2-) ion bound at the interface between three molecules. |
==About this Structure== | ==About this Structure== | ||
| - | 1HML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA, ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] Full crystallographic information is available from [http:// | + | 1HML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HML OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Lactose synthase]] | [[Category: Lactose synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Acharya, K | + | [[Category: Acharya, K R.]] |
[[Category: Ren, J.]] | [[Category: Ren, J.]] | ||
| - | [[Category: Stuart, D | + | [[Category: Stuart, D I.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: calcium-binding protein]] | [[Category: calcium-binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:02:48 2008'' |
Revision as of 11:02, 21 February 2008
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ALPHA_LACTALBUMIN POSSESSES A DISTINCT ZINC BINDING SITE
Overview
It has been proposed that the binding of Zn2+ to alpha-lactalbumin switches the conformation to one akin to a state intermediate in the folding of the protein. However, the high resolution x-ray crystal structure of human alpha-lactalbumin-Zn2+ complex at 1.7-A resolution (pH 7.6) does not reveal any significant change in conformation from the native state. The Zn2+ ion binds specifically in the "cleft" of alpha-lactalbumin (the region which forms the active site of the homologous protein lysozyme). This may suggest a possible role for Zn2+ binding in lactose synthase complex. The coordination of the Zn2+ ion involves a symmetry-related molecule in the crystal, the crystal contacts being stabilized by a SO4(2-) ion bound at the interface between three molecules.
About this Structure
1HML is a Single protein structure of sequence from Homo sapiens with , and as ligands. Active as Lactose synthase, with EC number 2.4.1.22 Full crystallographic information is available from OCA.
Reference
Alpha-lactalbumin possesses a distinct zinc binding site., Ren J, Stuart DI, Acharya KR, J Biol Chem. 1993 Sep 15;268(26):19292-8. PMID:8366079
Page seeded by OCA on Thu Feb 21 13:02:48 2008
Categories: Homo sapiens | Lactose synthase | Single protein | Acharya, K R. | Ren, J. | Stuart, D I. | CA | SO4 | ZN | Calcium-binding protein
