1ho3
From Proteopedia
(New page: 200px<br /><applet load="1ho3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ho3, resolution 2.5Å" /> '''CRYSTAL STRUCTURE ANA...) |
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| - | [[Image:1ho3.gif|left|200px]]<br /><applet load="1ho3" size=" | + | [[Image:1ho3.gif|left|200px]]<br /><applet load="1ho3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ho3, resolution 2.5Å" /> | caption="1ho3, resolution 2.5Å" /> | ||
'''CRYSTAL STRUCTURE ANALYSIS OF E. COLI L-ASPARAGINASE II (Y25F MUTANT)'''<br /> | '''CRYSTAL STRUCTURE ANALYSIS OF E. COLI L-ASPARAGINASE II (Y25F MUTANT)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Quasi-enantiomorphic crystals of the Y25F mutant of Escherichia coli | + | Quasi-enantiomorphic crystals of the Y25F mutant of Escherichia coli L-asparaginase and of the native Erwinia chrysanthemi L-asparaginase were obtained in the hexagonal space groups P6(5)22 and P6(1)22, respectively. The structures of these highly homologous enzymes were solved by molecular replacement and were refined with data extending to 2.2-2.5 A. These structures were compared with each other, as well as with other L-asparaginase structures previously observed with different crystal packing. It is concluded that the observed phenomenon, which is rare, was most likely to have arisen by chance. |
==About this Structure== | ==About this Structure== | ||
| - | 1HO3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ASP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] Full crystallographic information is available from [http:// | + | 1HO3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ASP:'>ASP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HO3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Kozak, M.]] | [[Category: Kozak, M.]] | ||
[[Category: Lubkowski, P.]] | [[Category: Lubkowski, P.]] | ||
| - | [[Category: Palm, J | + | [[Category: Palm, J G.]] |
[[Category: Wlodawer, A.]] | [[Category: Wlodawer, A.]] | ||
[[Category: ASP]] | [[Category: ASP]] | ||
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[[Category: leukemia]] | [[Category: leukemia]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:03:10 2008'' |
Revision as of 11:03, 21 February 2008
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CRYSTAL STRUCTURE ANALYSIS OF E. COLI L-ASPARAGINASE II (Y25F MUTANT)
Overview
Quasi-enantiomorphic crystals of the Y25F mutant of Escherichia coli L-asparaginase and of the native Erwinia chrysanthemi L-asparaginase were obtained in the hexagonal space groups P6(5)22 and P6(1)22, respectively. The structures of these highly homologous enzymes were solved by molecular replacement and were refined with data extending to 2.2-2.5 A. These structures were compared with each other, as well as with other L-asparaginase structures previously observed with different crystal packing. It is concluded that the observed phenomenon, which is rare, was most likely to have arisen by chance.
About this Structure
1HO3 is a Single protein structure of sequence from Escherichia coli with as ligand. Active as Asparaginase, with EC number 3.5.1.1 Full crystallographic information is available from OCA.
Reference
Structures of two highly homologous bacterial L-asparaginases: a case of enantiomorphic space groups., Jaskolski M, Kozak M, Lubkowski J, Palm G, Wlodawer A, Acta Crystallogr D Biol Crystallogr. 2001 Mar;57(Pt 3):369-77. PMID:11223513
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