2bip

From Proteopedia

Revision as of 12:11, 30 October 2007

HUMAN P53 CORE DOMAIN MUTANT M133L-H168R-V203A-N239Y-R249S-N268D

Overview

We have solved the crystal structures of three oncogenic mutants of the, core domain of the human tumor suppressor p53. The mutations were, introduced into a stabilized variant. The cancer hot spot mutation R273H, simply removes an arginine involved in DNA binding without causing, structural distortions in neighboring residues. In contrast, the, "structural" oncogenic mutations H168R and R249S induce substantial, structural perturbation around the mutation site in the L2 and L3 loops, respectively. H168R is a specific intragenic suppressor mutation for, R249S. When both cancer mutations are combined in the same molecule, Arg(168) mimics the role of Arg(249) in wild type, and the wild type, conformation is largely restored in both loops. Our structural and, biophysical data provide ... [(full description)]

About this Structure

2BIP is a [Single protein] structure of sequence from [Homo sapiens] with ZN as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structures of p53 cancer mutants and mechanism of rescue by second-site suppressor mutations., Joerger AC, Ang HC, Veprintsev DB, Blair CM, Fersht AR, J Biol Chem. 2005 Apr 22;280(16):16030-7. Epub 2005 Feb 9. PMID:15703170

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