1hv6
From Proteopedia
(New page: 200px<br /><applet load="1hv6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hv6, resolution 2.00Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1hv6.gif|left|200px]]<br /><applet load="1hv6" size=" | + | [[Image:1hv6.gif|left|200px]]<br /><applet load="1hv6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1hv6, resolution 2.00Å" /> | caption="1hv6, resolution 2.00Å" /> | ||
'''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.'''<br /> | '''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of A1-III from a Sphingomonas species A1 complexed with a | + | The structure of A1-III from a Sphingomonas species A1 complexed with a trisaccharide product (4-deoxy-l-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid) was determined by X-ray crystallography at 2.0 A with an R-factor of 0.16. The final model of the complex form comprising 351 amino acid residues, 245 water molecules, one sulfate ion and one trisaccharide product exhibited a C(alpha) r.m.s.d. value of 0.154 A with the reported apo form of the enzyme. The trisaccharide was bound in the active cleft at subsites -3 approximately -1 from the non-reducing end by forming several hydrogen bonds and van der Waals interactions with protein atoms. The catalytic residue was estimated to be Tyr246, which existed between subsites -1 and +1 based on a mannuronic acid model oriented at subsite +1. |
==About this Structure== | ==About this Structure== | ||
| - | 1HV6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sphingomonas_species Sphingomonas species] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Poly(beta-D-mannuronate)_lyase Poly(beta-D-mannuronate) lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.3 4.2.2.3] Full crystallographic information is available from [http:// | + | 1HV6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sphingomonas_species Sphingomonas species] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Poly(beta-D-mannuronate)_lyase Poly(beta-D-mannuronate) lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.3 4.2.2.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HV6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Miyake, O.]] | [[Category: Miyake, O.]] | ||
[[Category: Murata, K.]] | [[Category: Murata, K.]] | ||
| - | [[Category: Yoon, H | + | [[Category: Yoon, H J.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: alginate lyase]] | [[Category: alginate lyase]] | ||
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[[Category: trisaccharide complex]] | [[Category: trisaccharide complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:05:11 2008'' |
Revision as of 11:05, 21 February 2008
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CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.
Overview
The structure of A1-III from a Sphingomonas species A1 complexed with a trisaccharide product (4-deoxy-l-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid) was determined by X-ray crystallography at 2.0 A with an R-factor of 0.16. The final model of the complex form comprising 351 amino acid residues, 245 water molecules, one sulfate ion and one trisaccharide product exhibited a C(alpha) r.m.s.d. value of 0.154 A with the reported apo form of the enzyme. The trisaccharide was bound in the active cleft at subsites -3 approximately -1 from the non-reducing end by forming several hydrogen bonds and van der Waals interactions with protein atoms. The catalytic residue was estimated to be Tyr246, which existed between subsites -1 and +1 based on a mannuronic acid model oriented at subsite +1.
About this Structure
1HV6 is a Single protein structure of sequence from Sphingomonas species with as ligand. Active as Poly(beta-D-mannuronate) lyase, with EC number 4.2.2.3 Full crystallographic information is available from OCA.
Reference
Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution., Yoon HJ, Hashimoto W, Miyake O, Murata K, Mikami B, J Mol Biol. 2001 Mar 16;307(1):9-16. PMID:11243798
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