1hv8
From Proteopedia
(New page: 200px<br /><applet load="1hv8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hv8, resolution 3.0Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1hv8.jpg|left|200px]]<br /><applet load="1hv8" size=" | + | [[Image:1hv8.jpg|left|200px]]<br /><applet load="1hv8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1hv8, resolution 3.0Å" /> | caption="1hv8, resolution 3.0Å" /> | ||
'''CRYSTAL STRUCTURE OF A DEAD BOX PROTEIN FROM THE HYPERTHERMOPHILE METHANOCOCCUS JANNASCHII'''<br /> | '''CRYSTAL STRUCTURE OF A DEAD BOX PROTEIN FROM THE HYPERTHERMOPHILE METHANOCOCCUS JANNASCHII'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have determined the structure of a DEAD box putative RNA helicase from | + | We have determined the structure of a DEAD box putative RNA helicase from the hyperthermophile Methanococcus jannaschii. Like other helicases, the protein contains two alpha/beta domains, each with a recA-like topology. Unlike other helicases, the protein exists as a dimer in the crystal. Through an interaction that resembles the dimer interface of insulin, the amino-terminal domain's 7-strand beta-sheet is extended to 14 strands across the two molecules. Motifs conserved in the DEAD box family cluster in the cleft between domains, and many of their functions can be deduced by mutational data and by comparison with other helicase structures. Several lines of evidence suggest that motif III Ser-Ala-Thr may be involved in binding RNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1HV8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1HV8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HV8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Abelson, J | + | [[Category: Abelson, J N.]] |
[[Category: Li, H.]] | [[Category: Li, H.]] | ||
| - | [[Category: Story, R | + | [[Category: Story, R M.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: atpase]] | [[Category: atpase]] | ||
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[[Category: rna-binding protein]] | [[Category: rna-binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:05:16 2008'' |
Revision as of 11:05, 21 February 2008
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CRYSTAL STRUCTURE OF A DEAD BOX PROTEIN FROM THE HYPERTHERMOPHILE METHANOCOCCUS JANNASCHII
Overview
We have determined the structure of a DEAD box putative RNA helicase from the hyperthermophile Methanococcus jannaschii. Like other helicases, the protein contains two alpha/beta domains, each with a recA-like topology. Unlike other helicases, the protein exists as a dimer in the crystal. Through an interaction that resembles the dimer interface of insulin, the amino-terminal domain's 7-strand beta-sheet is extended to 14 strands across the two molecules. Motifs conserved in the DEAD box family cluster in the cleft between domains, and many of their functions can be deduced by mutational data and by comparison with other helicase structures. Several lines of evidence suggest that motif III Ser-Ala-Thr may be involved in binding RNA.
About this Structure
1HV8 is a Single protein structure of sequence from Methanocaldococcus jannaschii with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a DEAD box protein from the hyperthermophile Methanococcus jannaschii., Story RM, Li H, Abelson JN, Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1465-70. PMID:11171974
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