1hwq
From Proteopedia
(New page: 200px<br /><applet load="1hwq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hwq" /> '''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTR...) |
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| - | [[Image:1hwq.gif|left|200px]]<br /><applet load="1hwq" size=" | + | [[Image:1hwq.gif|left|200px]]<br /><applet load="1hwq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1hwq" /> | caption="1hwq" /> | ||
'''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP'''<br /> | '''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The VS ribozyme is a 154 nucleotide sequence found in certain natural | + | The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1HWQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1HWQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Dieckmann, T.]] | [[Category: Dieckmann, T.]] | ||
| - | [[Category: Flinders, J | + | [[Category: Flinders, J C.]] |
[[Category: a+c base pair]] | [[Category: a+c base pair]] | ||
[[Category: protonated adenine]] | [[Category: protonated adenine]] | ||
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[[Category: vs ribozyme]] | [[Category: vs ribozyme]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:05:34 2008'' |
Revision as of 11:05, 21 February 2008
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SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP
Overview
The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed.
About this Structure
1HWQ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA., Flinders J, Dieckmann T, J Mol Biol. 2001 May 11;308(4):665-79. PMID:11350168
Page seeded by OCA on Thu Feb 21 13:05:34 2008
