2biy
From Proteopedia
(New page: 200px<br /> <applet load="2biy" size="450" color="white" frame="true" align="right" spinBox="true" caption="2biy, resolution 1.95Å" /> '''STRUCTURE OF PDK1-S...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2BIY is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with SO4, ATP and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.37 2.7.1.37]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BIY OCA]]. | + | 2BIY is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with SO4, ATP and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_2.7.11.1 Transferred entry: 2.7.11.1]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.37 2.7.1.37]]. Structure known Active Site: BC6. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BIY OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Transferred entry: 2.7.11.1]] | ||
[[Category: Aalten, D.M.F.Van.]] | [[Category: Aalten, D.M.F.Van.]] | ||
[[Category: Alessi, D.R.]] | [[Category: Alessi, D.R.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:17:56 2007'' |
Revision as of 12:13, 30 October 2007
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STRUCTURE OF PDK1-S241A MUTANT KINASE DOMAIN
Overview
3-Phosphoinositide-dependent protein kinase-1 (PDK1) phosphorylates the, T-loop of several AGC (cAMP-dependent, cGMP-dependent, protein kinase C), family protein kinases, resulting in their activation. Previous structural, studies have revealed that the alpha C-helix, located in the small lobe of, the kinase domain of PDK1, is a key regulatory element, as it links a, substrate interacting site termed the hydrophobic motif (HM) pocket with, the phosphorylated Ser-241 in the T-loop. In this study we have, demonstrated by mutational analysis that interactions between the, phosphorylated Ser-241 and the alpha C-helix are not required for PDK1, activity or substrate binding through the HM-pocket but are necessary for, PDK1 to be activated or stabilized by a peptide that binds to this site., ... [(full description)]
About this Structure
2BIY is a [Single protein] structure of sequence from [Homo sapiens] with SO4, ATP and GOL as [ligands]. Active as [Transferred entry: 2.7.11.1], with EC number [2.7.1.37]. Structure known Active Site: BC6. Full crystallographic information is available from [OCA].
Reference
Role of T-loop phosphorylation in PDK1 activation, stability, and substrate binding., Komander D, Kular G, Deak M, Alessi DR, van Aalten DM, J Biol Chem. 2005 May 13;280(19):18797-802. Epub 2005 Mar 1. PMID:15741170
Page seeded by OCA on Tue Oct 30 14:17:56 2007
Categories: Homo sapiens | Single protein | Transferred entry: 2.7.11.1 | Aalten, D.M.F.Van. | Alessi, D.R. | Deak, M. | Komander, D. | Kular, G.S. | ATP | GOL | SO4 | Ac-helix | Agc kinase | Atp-binding | Phosphoinositide dependent protein kinase | Phosphorylation | Pi3-kinase signalling | Pif-pocket | Pka | Pkb | Serine-threonine-protein kinase | T-loop mutant | Transferase
