1i4w
From Proteopedia
(New page: 200px<br /><applet load="1i4w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i4w, resolution 2.60Å" /> '''THE CRYSTAL STRUCTUR...) |
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| - | [[Image:1i4w.gif|left|200px]]<br /><applet load="1i4w" size=" | + | [[Image:1i4w.gif|left|200px]]<br /><applet load="1i4w" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1i4w, resolution 2.60Å" /> | caption="1i4w, resolution 2.60Å" /> | ||
'''THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION'''<br /> | '''THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Although it is commonly accepted that binding of mitochondrial | + | Although it is commonly accepted that binding of mitochondrial transcription factor sc-mtTFB to the mitochondrial RNA polymerase is required for specific transcription initiation in Saccharomyces cerevisiae, its precise role has remained undefined. In the present work, the crystal structure of sc-mtTFB has been determined to 2.6 A resolution. The protein consists of two domains, an N-terminal alpha/beta-domain and a smaller domain made up of four alpha-helices. Contrary to previous predictions, sc-mtTFB does not resemble Escherichia coli sigma-factors but rather is structurally homologous to rRNA methyltransferase ErmC'. This suggests that sc-mtTFB functions as an RNA-binding protein, an observation standing in contradiction to the existing model, which proposed a direct interaction of sc-mtTFB with the mitochondrial DNA promoter. Based on the structure, we propose that the promoter specificity region is located on the mitochondrial RNA polymerase and that binding of sc-mtTFB indirectly mediates interaction of the core enzyme with the promoter site. |
==About this Structure== | ==About this Structure== | ||
| - | 1I4W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with XE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1I4W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=XE:'>XE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4W OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chen, C | + | [[Category: Chen, C J.]] |
| - | [[Category: Dailey, H | + | [[Category: Dailey, H A.]] |
| - | [[Category: Dailey, T | + | [[Category: Dailey, T A.]] |
| - | [[Category: Rose, J | + | [[Category: Rose, J P.]] |
| - | [[Category: Schubot, F | + | [[Category: Schubot, F D.]] |
| - | [[Category: Wang, B | + | [[Category: Wang, B C.]] |
[[Category: XE]] | [[Category: XE]] | ||
[[Category: mitochondrial transcription factor]] | [[Category: mitochondrial transcription factor]] | ||
[[Category: transcription initiation]] | [[Category: transcription initiation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:08:02 2008'' |
Revision as of 11:08, 21 February 2008
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THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION
Overview
Although it is commonly accepted that binding of mitochondrial transcription factor sc-mtTFB to the mitochondrial RNA polymerase is required for specific transcription initiation in Saccharomyces cerevisiae, its precise role has remained undefined. In the present work, the crystal structure of sc-mtTFB has been determined to 2.6 A resolution. The protein consists of two domains, an N-terminal alpha/beta-domain and a smaller domain made up of four alpha-helices. Contrary to previous predictions, sc-mtTFB does not resemble Escherichia coli sigma-factors but rather is structurally homologous to rRNA methyltransferase ErmC'. This suggests that sc-mtTFB functions as an RNA-binding protein, an observation standing in contradiction to the existing model, which proposed a direct interaction of sc-mtTFB with the mitochondrial DNA promoter. Based on the structure, we propose that the promoter specificity region is located on the mitochondrial RNA polymerase and that binding of sc-mtTFB indirectly mediates interaction of the core enzyme with the promoter site.
About this Structure
1I4W is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription., Schubot FD, Chen CJ, Rose JP, Dailey TA, Dailey HA, Wang BC, Protein Sci. 2001 Oct;10(10):1980-8. PMID:11567089
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