1i5c
From Proteopedia
(New page: 200px<br /><applet load="1i5c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i5c, resolution 1.90Å" /> '''STRUCTURE OF CHEA DO...) |
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| - | [[Image:1i5c.gif|left|200px]]<br /><applet load="1i5c" size=" | + | [[Image:1i5c.gif|left|200px]]<br /><applet load="1i5c" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1i5c, resolution 1.90Å" /> | caption="1i5c, resolution 1.90Å" /> | ||
'''STRUCTURE OF CHEA DOMAIN P4 IN COMPLEX WITH ADP'''<br /> | '''STRUCTURE OF CHEA DOMAIN P4 IN COMPLEX WITH ADP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | To probe the structural basis for protein histidine kinase (PHK) catalytic | + | To probe the structural basis for protein histidine kinase (PHK) catalytic activity and the prospects for PHK-specific inhibitor design, we report the crystal structures for the nucleotide binding domain of Thermotoga maritima CheA with ADP and three ATP analogs (ADPNP, ADPCP and TNP-ATP) bound with either Mg(2+) or Mn(2+). The conformation of ADPNP bound to CheA and related ATPases differs from that reported in the ADPNP complex of PHK EnvZ. Interactions of the active site with the nucleotide gamma-phosphate and its associated Mg(2+) ion are linked to conformational changes in an ATP-lid that could mediate recognition of the substrate domain. The inhibitor TNP-ATP binds CheA with its phosphates in a nonproductive conformation and its adenine and trinitrophenyl groups in two adjacent binding pockets. The trinitrophenyl interaction may be exploited for designing CheA-targeted drugs that would not interfere with host ATPases. |
==About this Structure== | ==About this Structure== | ||
| - | 1I5C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1I5C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5C OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
| - | [[Category: Bilwes, A | + | [[Category: Bilwes, A M.]] |
| - | [[Category: Crane, B | + | [[Category: Crane, B R.]] |
| - | [[Category: Croal, L | + | [[Category: Croal, L R.]] |
| - | [[Category: Quezada, C | + | [[Category: Quezada, C M.]] |
| - | [[Category: Simon, M | + | [[Category: Simon, M I.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: beta-alpha sandwich]] | [[Category: beta-alpha sandwich]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:08:09 2008'' |
Revision as of 11:08, 21 February 2008
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STRUCTURE OF CHEA DOMAIN P4 IN COMPLEX WITH ADP
Overview
To probe the structural basis for protein histidine kinase (PHK) catalytic activity and the prospects for PHK-specific inhibitor design, we report the crystal structures for the nucleotide binding domain of Thermotoga maritima CheA with ADP and three ATP analogs (ADPNP, ADPCP and TNP-ATP) bound with either Mg(2+) or Mn(2+). The conformation of ADPNP bound to CheA and related ATPases differs from that reported in the ADPNP complex of PHK EnvZ. Interactions of the active site with the nucleotide gamma-phosphate and its associated Mg(2+) ion are linked to conformational changes in an ATP-lid that could mediate recognition of the substrate domain. The inhibitor TNP-ATP binds CheA with its phosphates in a nonproductive conformation and its adenine and trinitrophenyl groups in two adjacent binding pockets. The trinitrophenyl interaction may be exploited for designing CheA-targeted drugs that would not interfere with host ATPases.
About this Structure
1I5C is a Single protein structure of sequence from Thermotoga maritima with as ligand. Full crystallographic information is available from OCA.
Reference
Nucleotide binding by the histidine kinase CheA., Bilwes AM, Quezada CM, Croal LR, Crane BR, Simon MI, Nat Struct Biol. 2001 Apr;8(4):353-60. PMID:11276258
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