1i9v
From Proteopedia
(New page: 200px<br /><applet load="1i9v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i9v, resolution 2.6Å" /> '''CRYSTAL STRUCTURE ANA...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1i9v.gif|left|200px]]<br /><applet load="1i9v" size=" | + | [[Image:1i9v.gif|left|200px]]<br /><applet load="1i9v" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1i9v, resolution 2.6Å" /> | caption="1i9v, resolution 2.6Å" /> | ||
'''CRYSTAL STRUCTURE ANALYSIS OF A TRNA-NEOMYCIN COMPLEX'''<br /> | '''CRYSTAL STRUCTURE ANALYSIS OF A TRNA-NEOMYCIN COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Aminoglycosides bind to RNA and interfere with its function, and it has | + | Aminoglycosides bind to RNA and interfere with its function, and it has been suggested that aminoglycoside binding to RNA displaces essential divalent metal ions. Here we demonstrate that addition of various aminoglycosides inhibited Pb2+-induced cleavage of yeast tRNA(Phe). Cocrystallization of yeast tRNA(Phe) and an aminoglycoside, neomycin B, resulted in crystals that diffracted to 2.6 A and the structure of the complex was solved by molecular replacement. The structure shows that the neomycin B binding site overlaps with known divalent metal ion binding sites in yeast tRNA(Phe), providing direct evidence for the hypothesis that aminoglycosides displace metal ions. Additionally, the neomycin B binding site overlaps with major determinants for Escherichia coli phenylalanyl-tRNA-synthetase. Here we present data demonstrating that addition of neomycin B inhibited aminoacylation of E. coli tRNA(Phe) in the mid microM range. Given that aminoglycoside and metal ion binding sites overlap, we discuss that aminoglycosides can be considered as 'metal mimics'. |
==About this Structure== | ==About this Structure== | ||
| - | 1I9V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG and NMY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1I9V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=NMY:'>NMY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9V OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Johansson, K.]] | [[Category: Johansson, K.]] | ||
| - | [[Category: Kirsebom, L | + | [[Category: Kirsebom, L A.]] |
| - | [[Category: Mikkelsen, N | + | [[Category: Mikkelsen, N E.]] |
[[Category: Virtanen, A.]] | [[Category: Virtanen, A.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
| Line 27: | Line 27: | ||
[[Category: yeast]] | [[Category: yeast]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:33 2008'' |
Revision as of 11:09, 21 February 2008
|
CRYSTAL STRUCTURE ANALYSIS OF A TRNA-NEOMYCIN COMPLEX
Overview
Aminoglycosides bind to RNA and interfere with its function, and it has been suggested that aminoglycoside binding to RNA displaces essential divalent metal ions. Here we demonstrate that addition of various aminoglycosides inhibited Pb2+-induced cleavage of yeast tRNA(Phe). Cocrystallization of yeast tRNA(Phe) and an aminoglycoside, neomycin B, resulted in crystals that diffracted to 2.6 A and the structure of the complex was solved by molecular replacement. The structure shows that the neomycin B binding site overlaps with known divalent metal ion binding sites in yeast tRNA(Phe), providing direct evidence for the hypothesis that aminoglycosides displace metal ions. Additionally, the neomycin B binding site overlaps with major determinants for Escherichia coli phenylalanyl-tRNA-synthetase. Here we present data demonstrating that addition of neomycin B inhibited aminoacylation of E. coli tRNA(Phe) in the mid microM range. Given that aminoglycoside and metal ion binding sites overlap, we discuss that aminoglycosides can be considered as 'metal mimics'.
About this Structure
1I9V is a Single protein structure of sequence from Saccharomyces cerevisiae with and as ligands. Full crystallographic information is available from OCA.
Reference
Aminoglycoside binding displaces a divalent metal ion in a tRNA-neomycin B complex., Mikkelsen NE, Johansson K, Virtanen A, Kirsebom LA, Nat Struct Biol. 2001 Jun;8(6):510-4. PMID:11373618
Page seeded by OCA on Thu Feb 21 13:09:33 2008
