1i9z

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1i9z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i9z, resolution 1.8&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
Line 1: Line 1:
-
[[Image:1i9z.jpg|left|200px]]<br /><applet load="1i9z" size="450" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1i9z.jpg|left|200px]]<br /><applet load="1i9z" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1i9z, resolution 1.8&Aring;" />
caption="1i9z, resolution 1.8&Aring;" />
'''CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 5-PHOSPHATASE DOMAIN (IPP5C) OF SPSYNAPTOJANIN IN COMPLEX WITH INOSITOL (1,4)-BISPHOSPHATE AND CALCIUM ION'''<br />
'''CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 5-PHOSPHATASE DOMAIN (IPP5C) OF SPSYNAPTOJANIN IN COMPLEX WITH INOSITOL (1,4)-BISPHOSPHATE AND CALCIUM ION'''<br />
==Overview==
==Overview==
-
Inositol polyphosphate 5-phosphatases are central to intracellular, processes ranging from membrane trafficking to Ca(2+) signaling, and, defects in this activity result in the human disease Lowe syndrome. The, 1.8 resolution structure of the inositol polyphosphate 5-phosphatase, domain of SPsynaptojanin bound to Ca(2+) and inositol (1,4)-bisphosphate, reveals a fold and an active site His and Asp pair resembling those of, several Mg(2+)-dependent nucleases. Additional loops mediate specific, inositol polyphosphate contacts. The 4-phosphate of inositol, (1,4)-bisphosphate is misoriented by 4.6 compared to the reactive geometry, observed in the apurinic/apyrimidinic endonuclease 1, explaining the, dephosphorylation site selectivity of the 5-phosphatases. Based on the, structure, a series of mutants are described that exhibit altered, substrate specificity providing general determinants for substrate, recognition.
+
Inositol polyphosphate 5-phosphatases are central to intracellular processes ranging from membrane trafficking to Ca(2+) signaling, and defects in this activity result in the human disease Lowe syndrome. The 1.8 resolution structure of the inositol polyphosphate 5-phosphatase domain of SPsynaptojanin bound to Ca(2+) and inositol (1,4)-bisphosphate reveals a fold and an active site His and Asp pair resembling those of several Mg(2+)-dependent nucleases. Additional loops mediate specific inositol polyphosphate contacts. The 4-phosphate of inositol (1,4)-bisphosphate is misoriented by 4.6 compared to the reactive geometry observed in the apurinic/apyrimidinic endonuclease 1, explaining the dephosphorylation site selectivity of the 5-phosphatases. Based on the structure, a series of mutants are described that exhibit altered substrate specificity providing general determinants for substrate recognition.
==About this Structure==
==About this Structure==
-
1I9Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] with CA and 2IP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I9Z OCA].
+
1I9Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=2IP:'>2IP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9Z OCA].
==Reference==
==Reference==
Line 14: Line 14:
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Guo, S.]]
[[Category: Guo, S.]]
-
[[Category: Hurley, J.H.]]
+
[[Category: Hurley, J H.]]
[[Category: Stolz, L.]]
[[Category: Stolz, L.]]
[[Category: Tsujishita, Y.]]
[[Category: Tsujishita, Y.]]
-
[[Category: York, J.D.]]
+
[[Category: York, J D.]]
[[Category: 2IP]]
[[Category: 2IP]]
[[Category: CA]]
[[Category: CA]]
Line 25: Line 25:
[[Category: spsynaptojanin]]
[[Category: spsynaptojanin]]
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:12:19 2007''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:35 2008''

Revision as of 11:09, 21 February 2008

Image:1i9z.jpg

1i9z, resolution 1.8Å

Drag the structure with the mouse to rotate

CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 5-PHOSPHATASE DOMAIN (IPP5C) OF SPSYNAPTOJANIN IN COMPLEX WITH INOSITOL (1,4)-BISPHOSPHATE AND CALCIUM ION

Overview

Inositol polyphosphate 5-phosphatases are central to intracellular processes ranging from membrane trafficking to Ca(2+) signaling, and defects in this activity result in the human disease Lowe syndrome. The 1.8 resolution structure of the inositol polyphosphate 5-phosphatase domain of SPsynaptojanin bound to Ca(2+) and inositol (1,4)-bisphosphate reveals a fold and an active site His and Asp pair resembling those of several Mg(2+)-dependent nucleases. Additional loops mediate specific inositol polyphosphate contacts. The 4-phosphate of inositol (1,4)-bisphosphate is misoriented by 4.6 compared to the reactive geometry observed in the apurinic/apyrimidinic endonuclease 1, explaining the dephosphorylation site selectivity of the 5-phosphatases. Based on the structure, a series of mutants are described that exhibit altered substrate specificity providing general determinants for substrate recognition.

About this Structure

1I9Z is a Single protein structure of sequence from Schizosaccharomyces pombe with and as ligands. Full crystallographic information is available from OCA.

Reference

Specificity determinants in phosphoinositide dephosphorylation: crystal structure of an archetypal inositol polyphosphate 5-phosphatase., Tsujishita Y, Guo S, Stolz LE, York JD, Hurley JH, Cell. 2001 May 4;105(3):379-89. PMID:11348594

Page seeded by OCA on Thu Feb 21 13:09:35 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1i9z"
Personal tools