1ia8
From Proteopedia
(New page: 200px<br /> <applet load="1ia8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ia8, resolution 1.7Å" /> '''THE 1.7 A CRYSTAL ST...) |
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| - | [[Image:1ia8.gif|left|200px]]<br /> | + | [[Image:1ia8.gif|left|200px]]<br /><applet load="1ia8" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ia8" size=" | + | |
caption="1ia8, resolution 1.7Å" /> | caption="1ia8, resolution 1.7Å" /> | ||
'''THE 1.7 A CRYSTAL STRUCTURE OF HUMAN CELL CYCLE CHECKPOINT KINASE CHK1'''<br /> | '''THE 1.7 A CRYSTAL STRUCTURE OF HUMAN CELL CYCLE CHECKPOINT KINASE CHK1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The checkpoint kinase Chk1 is an important mediator of cell cycle arrest | + | The checkpoint kinase Chk1 is an important mediator of cell cycle arrest following DNA damage. The 1.7 A resolution crystal structures of the human Chk1 kinase domain and its binary complex with an ATP analog has revealed an identical open kinase conformation. The secondary structure and side chain interactions stabilize the activation loop of Chk1 and enable kinase activity without phosphorylation of the catalytic domain. Molecular modeling of the interaction of a Cdc25C peptide with Chk1 has uncovered several conserved residues that are important for substrate selectivity. In addition, we found that the less conserved C-terminal region negatively impacts Chk1 kinase activity. |
==About this Structure== | ==About this Structure== | ||
| - | 1IA8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1IA8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IA8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chen, P.]] | [[Category: Chen, P.]] | ||
| - | [[Category: Connor, P | + | [[Category: Connor, P M.O.]] |
[[Category: Deng, Y.]] | [[Category: Deng, Y.]] | ||
| - | [[Category: Kan, C | + | [[Category: Kan, C C.Chen.]] |
[[Category: Lundgren, K.]] | [[Category: Lundgren, K.]] | ||
[[Category: Luo, C.]] | [[Category: Luo, C.]] | ||
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[[Category: protein kinase]] | [[Category: protein kinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:41 2008'' |
Revision as of 11:09, 21 February 2008
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THE 1.7 A CRYSTAL STRUCTURE OF HUMAN CELL CYCLE CHECKPOINT KINASE CHK1
Overview
The checkpoint kinase Chk1 is an important mediator of cell cycle arrest following DNA damage. The 1.7 A resolution crystal structures of the human Chk1 kinase domain and its binary complex with an ATP analog has revealed an identical open kinase conformation. The secondary structure and side chain interactions stabilize the activation loop of Chk1 and enable kinase activity without phosphorylation of the catalytic domain. Molecular modeling of the interaction of a Cdc25C peptide with Chk1 has uncovered several conserved residues that are important for substrate selectivity. In addition, we found that the less conserved C-terminal region negatively impacts Chk1 kinase activity.
About this Structure
1IA8 is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
The 1.7 A crystal structure of human cell cycle checkpoint kinase Chk1: implications for Chk1 regulation., Chen P, Luo C, Deng Y, Ryan K, Register J, Margosiak S, Tempczyk-Russell A, Nguyen B, Myers P, Lundgren K, Kan CC, O'Connor PM, Cell. 2000 Mar 17;100(6):681-92. PMID:10761933
Page seeded by OCA on Thu Feb 21 13:09:41 2008
Categories: Homo sapiens | Single protein | Chen, P. | Connor, P M.O. | Deng, Y. | Kan, C C.Chen. | Lundgren, K. | Luo, C. | Margosiak, S. | Myers, P. | Nguyen, B. | Register, J. | Ryan, K. | Tempczyk-Russell, A. | SO4 | Protein kinase
