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1ib1
From Proteopedia
(New page: 200px<br /> <applet load="1ib1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ib1, resolution 2.7Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1ib1.gif|left|200px]]<br /> | + | [[Image:1ib1.gif|left|200px]]<br /><applet load="1ib1" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ib1" size=" | + | |
caption="1ib1, resolution 2.7Å" /> | caption="1ib1, resolution 2.7Å" /> | ||
'''CRYSTAL STRUCTURE OF THE 14-3-3 ZETA:SEROTONIN N-ACETYLTRANSFERASE COMPLEX'''<br /> | '''CRYSTAL STRUCTURE OF THE 14-3-3 ZETA:SEROTONIN N-ACETYLTRANSFERASE COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Serotonin N-acetyltransferase (AANAT) controls the daily rhythm in | + | Serotonin N-acetyltransferase (AANAT) controls the daily rhythm in melatonin synthesis. When isolated from tissue, AANAT copurifies with isoforms epsilon and zeta of 14-3-3. We have determined the structure of AANAT bound to 14-3-3zeta, an association that is phosphorylation dependent. AANAT is bound in the central channel of the 14-3-3zeta dimer, and is held in place by extensive interactions both with the amphipathic phosphopeptide binding groove of 14-3-3zeta and with other parts of the central channel. Thermodynamic and activity measurements, together with crystallographic analysis, indicate that binding of AANAT by 14-3-3zeta modulates AANAT's activity and affinity for its substrates by stabilizing a region of AANAT involved in substrate binding. |
==About this Structure== | ==About this Structure== | ||
| - | 1IB1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] with COT as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Aralkylamine_N-acetyltransferase Aralkylamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.87 2.3.1.87] Full crystallographic information is available from [http:// | + | 1IB1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] with <scene name='pdbligand=COT:'>COT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Aralkylamine_N-acetyltransferase Aralkylamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.87 2.3.1.87] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IB1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Ganguly, S.]] | [[Category: Ganguly, S.]] | ||
[[Category: Ghirlando, R.]] | [[Category: Ghirlando, R.]] | ||
| - | [[Category: Klein, D | + | [[Category: Klein, D C.]] |
[[Category: Obsil, T.]] | [[Category: Obsil, T.]] | ||
[[Category: COT]] | [[Category: COT]] | ||
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[[Category: signal transduction]] | [[Category: signal transduction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:55 2008'' |
Revision as of 11:09, 21 February 2008
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CRYSTAL STRUCTURE OF THE 14-3-3 ZETA:SEROTONIN N-ACETYLTRANSFERASE COMPLEX
Overview
Serotonin N-acetyltransferase (AANAT) controls the daily rhythm in melatonin synthesis. When isolated from tissue, AANAT copurifies with isoforms epsilon and zeta of 14-3-3. We have determined the structure of AANAT bound to 14-3-3zeta, an association that is phosphorylation dependent. AANAT is bound in the central channel of the 14-3-3zeta dimer, and is held in place by extensive interactions both with the amphipathic phosphopeptide binding groove of 14-3-3zeta and with other parts of the central channel. Thermodynamic and activity measurements, together with crystallographic analysis, indicate that binding of AANAT by 14-3-3zeta modulates AANAT's activity and affinity for its substrates by stabilizing a region of AANAT involved in substrate binding.
About this Structure
1IB1 is a Protein complex structure of sequences from Homo sapiens and Ovis aries with as ligand. Active as Aralkylamine N-acetyltransferase, with EC number 2.3.1.87 Full crystallographic information is available from OCA.
Reference
Crystal structure of the 14-3-3zeta:serotonin N-acetyltransferase complex. a role for scaffolding in enzyme regulation., Obsil T, Ghirlando R, Klein DC, Ganguly S, Dyda F, Cell. 2001 Apr 20;105(2):257-67. PMID:11336675
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